Anti-TERF2IP Antibody (CAB15163)

Antibody Name:	Anti-TERF2IP Antibody
Antibody SKU:	CAB15163
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TERF2IP (NP_061848.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	HeLa, NCI-H460

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TERF2IP (NP_061848.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAEA MDLG KDPN GPTH SSTL FVRD DGSS MSFY VRPS PAKR RLST LILH GGGT VCRV QEPG AVLL AQPG EALA EASG DFIS TQYI LDCV ERNE RLEL EAYR LGPA SAAD TGSE AKPG ALAE GAAE PEPQ RHAG RIAF TDAD DVAI LTYV KENA RSPS SVTG NALW KAME KSSL TQHS WQSL KDRY LKHL RGQE HKYL LGDA PVSP SSQK LKRK AEED PEAA DSGE PQNK RTPD LPEE EYVK EEIQ ENEE AVKK MLVE ATRE FEEV VVDE SPPD FEIH ITMC
Gene ID:	54386
Uniprot:	Q9NYB0
Cellular Location:	Chromosome, Cytoplasm, Nucleus, telomere
Calculated MW:	44kDa
Observed MW:	55kDa

Synonyms:	TERF2IP, DRIP5, RAP1
Background:	The gene encodes a protein that is part of a complex involved in telomere length regulation. Pseudogenes are present on chromosomes 5 and 22.

UniProt Protein Function:	TERF2IP: Acts both as a regulator of telomere function and as a transcription regulator. Involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. Instead, it is required to negatively regulate telomere recombination and is essential for repressing homology-directed repair (HDR), which can affect telomere length. Does not bind DNA directly: recruited to telomeric double-stranded 5'-TTAGGG-3' repeats via its interaction with TERF2. Independently of its function in telomeres, also acts as a transcription regulator: recruited to extratelomeric 5'- TTAGGG-3' sites via its association with TERF2 or other factors, and regulates gene expression. When cytoplasmic, associates with the I-kappa-B-kinase (IKK) complex and acts as a regulator of the NF-kappa-B signaling by promoting IKK-mediated phosphorylation of RELA/p65, leading to activate expression of NF-kappa-B target genes. Associates with the I-kappa-B-kinase (IKK) core complex, composed of CHUK, IKBKB and IKBKG. Homodimer. Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP ACD and POT1. Interacts with TERF2; the interaction is direct. Does not interact with TERF1. Interacts with SLX4/BTBD12. Ubiquitous. Highly expressed. Belongs to the RAP1 family.
UniProt Protein Details:	Protein type:DNA replication Chromosomal Location of Human Ortholog: 16q23.1 Cellular Component: chromosome, telomeric region; cytoplasm; Mre11 complex; nuclear chromosome; nuclear chromosome, telomeric region; nuclear telomere cap complex; nucleoplasm; nucleus Molecular Function:protein binding; telomeric DNA binding Biological Process: activation of NF-kappaB transcription factor; negative regulation of DNA recombination at telomere; negative regulation of telomere maintenance; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of peptidyl-serine phosphorylation; protection from non-homologous end joining at telomere; regulation of transcription, DNA-dependent; telomere capping; telomere maintenance; telomere maintenance via telomerase
NCBI Summary:	The gene encodes a protein that is part of a complex involved in telomere length regulation. Pseudogenes are present on chromosomes 5 and 22. [provided by RefSeq, Apr 2010]
UniProt Code:	Q9NYB0
NCBI GenInfo Identifier:	21542267
NCBI Gene ID:	54386
NCBI Accession:	Q9NYB0.1
UniProt Secondary Accession:	Q9NYB0,Q4W4Y2, Q8WYZ3, Q9NWR2, B4DQN4,
UniProt Related Accession:	Q9NYB0
Molecular Weight:	44,260 Da
NCBI Full Name:	Telomeric repeat-binding factor 2-interacting protein 1
NCBI Synonym Full Names:	TERF2 interacting protein
NCBI Official Symbol:	TERF2IP
NCBI Official Synonym Symbols:	RAP1; DRIP5
NCBI Protein Information:	telomeric repeat-binding factor 2-interacting protein 1
UniProt Protein Name:	Telomeric repeat-binding factor 2-interacting protein 1
UniProt Synonym Protein Names:	Dopamine receptor-interacting protein 5; Repressor/activator protein 1 homolog; RAP1 homolog; hRap1
Protein Family:	Telomeric repeat-binding factor 2-interacting protein
UniProt Gene Name:	TERF2IP
UniProt Entry Name:	TE2IP_HUMAN

	Western blot analysis of extracts of various cell lines, using TERF2IP Rabbit pAb (CAB15163) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using TERF2IP antibody (CAB15163) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-TERF2IP Antibody (CAB7981)

TERF2IP Antibody (PACO01410)

TERF2IP Antibody (PACO12697)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)