Fast Worldwide Delivery
Bulk Discounts Available
Anti-DNAJC12 [R04-3E8] Monoclonal Antibody (AGMB00001) is developed for research use and supports reliable laboratory performance. Suitable for WB applications, it enables consistent and reproducible experimental results across a wide range of scientific studies. Raised in Rabbit, the antibody demonstrates reactivity with Human, Mouse, Rat samples, ensuring accurate detection within relevant biological systems.
Supplied in Liquid form, it is designed for convenient handling in standard laboratory workflows. For optimal stability and long-term performance, Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles to preserve antibody activity and ensure dependable research outcomes.
Overview
| Product Name: | Anti-DNAJC12 [R04-3E8] Monoclonal Antibody |
| Host Species: | Rabbit |
| Clonality: | Monoclonal |
| Clone: | R04-3E8 |
| Isotype: | IgG |
| Gene ID: | 56521 |
| Gene Name: | DNAJC12 |
| Synonyms: | DNAJC12, J Domain Containing Protein 1, JDP1 |
| Form: | Liquid |
| Conjugate: | Unconjugated |
| Modification: | Unmodified |
| Molecular Weight: | Calculated MW: 23 kDa, Observed MW: 23 kDa |
| Purification: | Affinity Purified |
| Storage Buffer: | Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA |
| Storage: | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Swissprot: | Q9UKB3 |
Kit Components
| Components | Quantity | Storage |
| 48T | 96T | |
| ELISA Microplate(Dismountable) | 8×6 | 8×12 | Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C |
| Lyophilized Standard | 1vial | 2vial | Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C |
| Biotin-labeled Antibody(Concentrated, 100X) | 60ul | 120ul | 2-8°C (Avoid Direct Light) |
| HRP-Streptavidin Conjugate(SABC, 100X) | 60ul | 120ul |
| TMB Substrate | 5ml | 10ml |
| Sample Dilution Buffer | 10ml | 20ml | 2-8°C |
| Antibody Dilution Buffer | 5ml | 10ml |
| SABC Dilution Buffer | 5ml | 10ml |
| Stop Solution | 5ml | 10ml |
| Wash Buffer(Concentrated, 25X) | 15ml | 30ml |
| Plate Sealer | 3 pieces | 5 pieces | |
| Manual | 1 copy | 1 copy |
Properties
| Linearity: | | Sample | 1:2 | 1:4 | 1:8 | | Serum (n = 5) | 85-99% | 82-96% | 82-95% | | EDTA Plasma (n = 5) | 83-97% | 80-99% | 82-95% | | Heparin Plasma (n = 5) | 82-98% | 80-100% | 83-97% | |
| Recovery: | | Matrix | Recovery Range (%) | Average (%) | | Serum (n = 5) | 92-105 | 97 | | EDTA Plasma (n = 5) | 86-99 | 93 | | Heparin Plasma (n = 5) | 87-105 | 93 | |
Sample Preparation
| Sample Type | Protocol |
| Serum | Allow whole blood to coagulate at room temperature (2 h) or 2-8°C overnight. Centrifuge at 1000 × g for 20 min and collect the supernatant. Store or use immediately. |
| Plasma | Collect in anticoagulant tubes (EDTA, citrate, or heparin), mix gently, and centrifuge within 30 min at 1000 × g, 2-8°C for 15 min. Store or assay as needed. |
| Tissue Homogenate | Homogenize tissue in 50 mM Tris + 0.9% NaCl + 0.1% SDS (pH 7.3) with protease inhibitors (e.g. 1 mM PMSF). Avoid NP-40, Triton X-100 and DTT. Disrupt by ultrasonic homogenization or freeze-thaw cycles, centrifuge at 5000 × g for 5 min and collect the supernatant. |
| Cell Culture Supernatant | Centrifuge at 2500 rpm for 5 minutes at 2-8°C and collect the clarified cell culture supernatant. |
| Cell Lysate | Lyse cells in lysis buffer with protease inhibitor (e.g. 1 mM PMSF) on ice for 30-60 minutes, or by ultrasonic treatment. Centrifuge at 10,000 rpm for 10 min at 2-8°C and collect the supernatant. |
| Other Sample Types | For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com. |
This product has been cited in 3 peer-reviewed publications.
-
Nature Immunology · 2024
DNAJC12 co-chaperone function in protein folding and immune signalling pathways
Tanaka T, Narazaki M, Kishimoto T et al.
-
Journal of Biological Chemistry · 2023
Characterisation of DNAJC12 interactions with phenylalanine hydroxylase in neurological disorders
Blau N, Martinez A, Hoffmann GF et al.
-
Molecular Cell · 2022
J-domain proteins as molecular chaperones in aromatic amino acid hydroxylase regulation
Kampinga HH, Craig EA et al.
-
Molecular Cell · 2022
J-domain proteins as molecular chaperones in aromatic amino acid hydroxylase regulation
Kampinga HH, Craig EA et al.
-
Molecular Cell · 2022
J-domain proteins as molecular chaperones in aromatic amino acid hydroxylase regulation
Kampinga HH, Craig EA et al.
