Description
TGEV RBD Recombinant Protein (N-His)
TGEV RBD Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Pro521-Pro667, with an N-terminal His tag. Predicted molecular weight: 18.84 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Spike glycoprotein is a ~160 kDa protein. S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. For research use only.
Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain.
| Product Name: | TGEV RBD Recombinant Protein (N-His) |
| SKU: | GXRP12972 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | TGEV |
| Synonyms: | E2, Peplomer protein, S, S glycoprotein, Spike glycoprotein |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Pro521-Pro667 |
| Calculated MW: | 18.84 kDa |
| UniProt: | P07946 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
