The THUMPD3 Antibody (CAB10407) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the THUMPD3 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for studies in molecular biology and RNA modification research.THUMPD3 is an essential player in the RNA modification pathway, specifically in the formation of 2'-O-methylated pseudouridines in tRNA molecules.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
THUMPD3 Antibody
SKU:
CAB10407
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
THUMPD3
Positive Sample:
LO2, A-549, MCF7
Cellular Localization:
Cytoplasm, Cytosol, Nucleolus.
Calculated MW:
57kDa
Observed MW:
57kDa
Predicted to enable tRNA (guanine) methyltransferase activity. Predicted to be involved in tRNA methylation. Located in cytosol and nucleolus.
Purification Method
Affinity purification
Gene ID
25917
RRID
AB_2757955
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using THUMPD3 Rabbit pAb (CAB10407) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using THUMPD3 Rabbit pAb (CAB10407) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat ovary using THUMPD3 Rabbit pAb (CAB10407) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
