Anti-TIA1 Antibody (CAB12517)

Antibody Name:	Anti-TIA1 Antibody
Antibody SKU:	CAB12517
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human TIA1 (NP_071505.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, 293T, SKOV3, HeLa, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human TIA1 (NP_071505.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEDE MPKT LYVG NLSR DVTE ALIL QLFS QIGP CKNC KMIM DTAG NDPY CFVE FHEH RHAA AALA AMNG RKIM GKEV KVNW ATTP SSQK KDTS SSTV VSTQ RSQD HFHV FVGD LSPE ITTE DIKA AFAP FGRI SDAR VVKD MATG KSKG YGFV SFFN KWDA ENAI QQMG GQWL GGRQ IRTN WATR KPPA PKST Y
Gene ID:	7072
Uniprot:	P31483
Cellular Location:	Cytoplasmic granule, Nucleus
Calculated MW:	24kDa/41kDa/42kDa
Observed MW:	43kDa

Synonyms:

TIA1, TIA-1, WDM

Background:

The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature.

UniProt Protein Function:	TIA1: Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Translation; RNA-binding Chromosomal Location of Human Ortholog: 2p13 Cellular Component: nucleoplasm; stress granule; cytoplasm Molecular Function:protein binding; nucleotide binding; AU-rich element binding; poly(A) binding Biological Process: regulation of nuclear mRNA splicing, via spliceosome; apoptosis; negative regulation of translation; negative regulation of cytokine biosynthetic process Disease: Welander Distal Myopathy
NCBI Summary:	The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms has been found for this gene. [provided by RefSeq, May 2017]
UniProt Code:	P31483
NCBI GenInfo Identifier:	206729905
NCBI Gene ID:	7072
NCBI Accession:	P31483.3
UniProt Related Accession:	P31483
Molecular Weight:	43kDa
NCBI Full Name:	Nucleolysin TIA-1 isoform p40
NCBI Synonym Full Names:	TIA1 cytotoxic granule associated RNA binding protein
NCBI Official Symbol:	TIA1
NCBI Official Synonym Symbols:	WDM; TIA-1
NCBI Protein Information:	nucleolysin TIA-1 isoform p40; nucleolysin TIA-1
UniProt Protein Name:	Nucleolysin TIA-1 isoform p40
UniProt Synonym Protein Names:	RNA-binding protein TIA-1; T-cell-restricted intracellular antigen-1; TIA-1; p40-TIA-1
Protein Family:	Nucleolysin
UniProt Gene Name:	TIA1
UniProt Entry Name:	TIA1_HUMAN

	Western blot analysis of extracts of various cell lines, using TIA1 antibody (CAB12517) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded human tonsil using TIA1 antibody (CAB12517) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using TIA1 antibody (CAB12517) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-TIA1 Antibody (CAB12523)

Anti-TIA1 Antibody (CAB6237)

TIA1 Antibody (PACO12749)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)