The TK Polyclonal Antibody (PACO22733) is a crucial tool for researchers studying TK, a key enzyme involved in cellular metabolism and DNA synthesis. This antibody, produced in rabbits, exhibits high specificity for TK in various samples and has been validated for use in applications such as Western blotting.TK, also known as thymidine kinase, is essential for the replication of DNA and is often targeted in cancer therapy due to its role in cell proliferation. By targeting TK, researchers can gain a better understanding of cellular processes involved in cancer development and potentially identify new therapeutic targets.
The TK Polyclonal Antibody is a valuable resource for researchers investigating the role of TK in cancer biology, as well as those studying cellular metabolism and DNA replication. Its ability to specifically bind to TK enables precise detection and analysis of this important enzyme in a variety of cell types, making it an indispensable tool in cancer research and drug development efforts.
Antibody Name:
TK Antibody (PACO22733)
Antibody SKU:
PACO22733
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from internal of human KITH_HHV1.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from HeLa cells, using KITH_HHV1 antibody.
Background:
In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as nerve cells that do not replicate their genome By similarity.
Synonyms:
Thymidine kinase; EC=2.7.1.21; TK; UL23;
UniProt Protein Function:
Function: In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as nerve cells that do not replicate their genome By similarity.Catalytic activity: ATP + thymidine = ADP + thymidine 5'-phosphate.Subunit structure: Homodimer By similarity.Biotechnological use: Used in molecular biology as a selectable marker to identify transfected eukaryotic cells. Used in cancer suicide gene therapy to selectively kill transformed cells.Miscellaneous: Phosphorylates and thereby activates certain drugs used to treat herpes simplex infections like acyclovir (ACV), valaciclovir, and famciclovir to a toxic form, that leads to successful suppression of the infection, while the uninfected cell does not have this ability because it lacks TK. Mutations in thymidine kinase may induce HHV resistance to antiviral therapies in immunocompromised patients. The most frequently observed resistant strains are unable to express TK and are avirulent in animal models of disease. Resistance may be acquired less frequently by selecting variants which no longer recognize ACV or ACV triphosphate as substrates but which retain normal functions.The sequence shown is that of strain TAS.Sequence similarities: Belongs to the herpesviruses thymidine kinase family.
