Anti-TK1 Antibody (CAB5612)

Antibody Name:	Anti-TK1 Antibody
Antibody SKU:	CAB5612
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-234 of human TK1 (NP_003249.3).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Mouse spleen, Mouse ovary

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-234 of human TK1 (NP_003249.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSCI NLPT VLPG SPSK TRGQ IQVI LGPM FSGK STEL MRRV RRFQ IAQY KCLV IKYA KDTR YSSS FCTH DRNT MEAL PACL LRDV AQEA LGVA VIGI DEGQ FFPD IVEF CEAM ANAG KTVI VAAL DGTF QRKP FGAI LNLV PLAE SVVK LTAV CMEC FREA AYTK RLGT EKEV EVIG GADK YHSV CRLC YFKK ASGQ PAGP DNKE NCPV PGKP GEAV AARK LFAP QQIL QCSP AN
Gene ID:	7083
Uniprot:	P04183
Cellular Location:	Cytoplasm
Calculated MW:	25kDa
Observed MW:	30kDa

Synonyms:

TK1, TK2

Background:

The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers.

UniProt Protein Function:	TK: cytosolic thymidine kinase. Phosphorylated during mitosis. Its enzymatic activity is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.
UniProt Protein Details:	Protein type:EC 2.7.1.21; Xenobiotic Metabolism - drug metabolism - other enzymes; Nucleotide Metabolism - pyrimidine; Kinase, other Chromosomal Location of Human Ortholog: 17q23.2-q25.3 Cellular Component: cytosol Molecular Function:identical protein binding; nucleoside kinase activity; zinc ion binding; thymidine kinase activity; ATP binding Biological Process: response to nutrient levels; skeletal muscle cell proliferation; nucleobase, nucleoside and nucleotide metabolic process; response to toxin; liver development; protein homotetramerization; fetal process involved in parturition; deoxyribonucleoside monophosphate biosynthetic process; response to cortisol stimulus; pyrimidine base metabolic process; gut development; response to copper ion; nucleotide biosynthetic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; thymidine metabolic process; pyrimidine nucleoside salvage; phosphorylation
UniProt Code:	P04183
NCBI GenInfo Identifier:	23503074
NCBI Gene ID:	7083
NCBI Accession:	P04183.2
UniProt Secondary Accession:	P04183,Q969V0, Q9UMG9, B2RC58,
UniProt Related Accession:	P04183
Molecular Weight:	234
NCBI Full Name:	Thymidine kinase, cytosolic
NCBI Synonym Full Names:	thymidine kinase 1, soluble
NCBI Official Symbol:	TK1
NCBI Official Synonym Symbols:	TK2
NCBI Protein Information:	thymidine kinase, cytosolic; thymidine kinase-1; thymidine kinase 1 soluble isoform
UniProt Protein Name:	Thymidine kinase, cytosolic
Protein Family:	Thymidine kinase
UniProt Gene Name:	TK1
UniProt Entry Name:	KITH_HUMAN

	Western blot analysis of extracts of various cell lines, using TK1 antibody (CAB5612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded Rat liver using TK1 antibody (CAB5612) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human esophageal using TK1 antibody (CAB5612) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-Thymidine Kinase 1 Antibody (CAB13612)	Human Thymidine Kinase 1 / TK1 ELISA Kit
Phospho-TK1 (S13) Antibody (PACO05212)	Human TK1 (Thymidine Kinase 1, Soluble) CLIA Kit (HUES01276)
TK1 Antibody (PACO05213)	Human Thymidine kinase, cytosolic (TK1) ELISA Kit
TK1 Antibody (PACO05214)	TK (Phospho-Ser13) Colorimetric Cell-Based ELISA Kit
TK1 Antibody (PACO12772)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)