Western blot analysis of various lysates, using TLE4 Rabbit mAb (CAB23693) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The TLE4 Monoclonal Antibody (CAB23693) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, monoclonal in nature, is highly specific for human samples and is validated for use in immunohistochemistry applications. TLE4, a member of the Groucho/TLE family of proteins, is known to interact with various transcription factors and modulate gene expression in a context-dependent manner. Its role in regulating cell proliferation, differentiation, and apoptosis makes it a key player in developmental biology and cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
TLE4 Monoclonal Antibody
SKU:
CAB23693
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC61324
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Predicted to enable transcription corepressor activity. Predicted to be involved in negative regulation of canonical Wnt signaling pathway. Predicted to act upstream of or within Wnt signaling pathway; cellular response to leukemia inhibitory factor; and negative regulation of transcription by RNA polymerase II. Located in nucleoplasm. Part of beta-catenin-TCF complex.
Purification Method
Affinity purification
Gene ID
7091
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TLE4 Rabbit mAb (CAB23693) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
