TNF-Alpha Rabbit Polyclonal Antibody (CAB11534)

(No reviews yet) Write a Review
SKU:
CAB11534
  • Western blot analysis of extracts of various cell lines, using TNF-? antibody (CAB11534). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
  • Immunohistochemistry of paraffin-embedded human stomach using TNF-? antibody (CAB11534) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of L929 cells using TNF alpha antibody (CAB11534) at dilution of 1:100. Blue: DAPI for nuclear staining.
€99 - €249

Description

Technical Manual

TNF-Alpha Rabbit Polyclonal Antibody

Product Name:TNF-Alpha Rabbit Polyclonal Antibody
Product SKU:CAB11534
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Immunogen:A synthetic peptide of human TNF
Sequence:

Email for sequence

Gene ID:7124
Swiss Prot:P01375
Synonyms:TNF; DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F; TNF alpha
Calculated MW:25kDa
Observed MW:21kDa
Reactivity:Human, Mouse, Rat
Application:WB IHC IF
Recommended Dilution:WB 1:1000 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry Immunofluorescence 
Positive Samples:HeLa, Raji, Mouse thymus, Mouse spleen
Cellular Location:Cell membrane, Membrane, Secreted, Single-pass type II membrane protein, Single-pass type II membrane protein

Product Images

TNF-Alpha Rabbit Polyclonal Antibody (CAB11534)

Western blot analysis of extracts of various cell lines, using TNF-Alpha antibody (CAB11534). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

TNF-Alpha Rabbit Polyclonal Antibody (CAB11534)

Immunohistochemistry of paraffin-embedded human stomach using TNF-Alpha antibody (CAB11534) at dilution of 1:100 (40x lens).

TNF-Alpha Rabbit Polyclonal Antibody (CAB11534)

Immunofluorescence analysis of L929 cells using TNF alpha antibody (CAB11534) at dilution of 1:100. Blue: DAPI for nuclear staining.

Uniprot Information

UniProt Protein Function:TNF-a: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Homotrimer. Interacts with SPPL2B. Belongs to the tumor necrosis factor family.
UniProt Protein Details:

Protein type:Membrane protein, integral; Motility/polarity/chemotaxis; Apoptosis; Cytokine

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular space; recycling endosome; cell surface; integral to plasma membrane; extracellular region; plasma membrane; external side of plasma membrane; phagocytic cup; lipid raft

Molecular Function:identical protein binding; protein binding; protease binding; cytokine activity; tumor necrosis factor receptor binding

Biological Process: positive regulation of JNK activity; extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; positive regulation of NFAT protein import into nucleus; activation of MAPK activity; positive regulation of osteoclast differentiation; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; positive regulation of caspase activity; positive regulation of NF-kappaB import into nucleus; osteoclast differentiation; positive regulation of translational initiation by iron; positive regulation of membrane protein ectodomain proteolysis; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; tumor necrosis factor-mediated signaling pathway; positive regulation of phagocytosis; negative regulation of interleukin-6 production; JNK cascade; negative regulation of osteoblast differentiation; positive regulation of action potential; regulation of immunoglobulin secretion; embryonic gut development; negative regulation of protein complex disassembly; positive regulation of cytokine production; response to drug; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of heterotypic cell-cell adhesion; positive regulation of mitosis; response to virus; positive regulation of interleukin-8 biosynthetic process; glucose metabolic process; positive regulation of interleukin-6 production; negative regulation of fat cell differentiation; positive regulation of chemokine production; negative regulation of cytokine secretion during immune response; positive regulation of protein transport; detection of mechanical stimulus involved in sensory perception of pain; cell activation; defense response to Gram-positive bacterium; induction of apoptosis via death domain receptors; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of L-glutamate transport; response to activity; negative regulation of transcription, DNA-dependent; skeletal muscle contraction; sequestering of triacylglycerol; positive regulation of smooth muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-18 production; chronic inflammatory response to antigenic stimulus; response to salt stress; positive regulation of synaptic transmission; positive regulation of hair follicle development; negative regulation of cell proliferation; response to radiation; negative regulation of lipid catabolic process; positive regulation of neuron apoptosis; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; positive regulation of chronic inflammatory response to antigenic stimulus; inflammatory response; regulation of I-kappaB kinase/NF-kappaB cascade; caspase activation; positive regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of protein complex disassembly; transformed cell apoptosis; MAPKKK cascade; calcium-mediated signaling; positive regulation of peptidyl-serine phosphorylation; humoral immune response; positive regulation of protein kinase B signaling cascade; positive regulation of interferon-gamma production; negative regulation of glucose import; positive regulation of programmed cell death; positive regulation of chemokine biosynthetic process; positive regulation of protein complex assembly; negative regulation of viral genome replication; protein import into nucleus, translocation; positive regulation of protein kinase activity; response to hypoxia; positive regulation of fever; activation of MAPKKK activity; receptor biosynthetic process; positive regulation of protein amino acid phosphorylation; leukocyte tethering or rolling; negative regulation of myoblast differentiation; regulation of insulin secretion; positive regulation of cytokine secretion

Disease: Asthma, Susceptibility To; Migraine With Or Without Aura, Susceptibility To, 1; Malaria, Susceptibility To

NCBI Summary:This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
UniProt Code:P01375
NCBI GenInfo Identifier:135934
NCBI Gene ID:7124
NCBI Accession:P01375.1
UniProt Secondary Accession:P01375,O43647, Q9P1Q2, Q9UIV3,
UniProt Related Accession:P01375
Molecular Weight:25,644 Da
NCBI Full Name:Tumor necrosis factor
NCBI Synonym Full Names:tumor necrosis factor
NCBI Official Symbol:TNF 
NCBI Official Synonym Symbols:DIF; TNFA; TNFSF2; TNF-alpha 
NCBI Protein Information:tumor necrosis factor; TNF-a; cachectin; APC1 protein; TNF, monocyte-derived; TNF, macrophage-derived; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2
UniProt Protein Name:Tumor necrosis factor
UniProt Synonym Protein Names:Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
Protein Family:Tumor necrosis factor
UniProt Gene Name:TNF 
UniProt Entry Name:TNFA_HUMAN
View AllClose

Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
View AllClose

0 Reviews

View AllClose