Anti-TNNI3 Antibody (CAB6995)
- SKU:
- CAB6995
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-TNNI3 Antibody |
| Antibody SKU: | CAB6995 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human TNNI3 (NP_000354.4). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse heart, Rat heart, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human TNNI3 (NP_000354.4). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MADG SSDA AREP RPAP APIR RRSS NYRA YATE PHAK KKSK ISAS RKLQ LKTL LLQI AKQE LERE AEER RGEK GRAL STRC QPLE LAGL GFAE LQDL CRQL HARV DKVD EERY DIEA KVTK NITE IADL TQKI FDLR GKFK RPTL RRVR ISAD AMMQ ALLG ARAK ESLD LRAH LKQV KKED TEKE NREV GDWR KNID ALSG MEGR KKKF ES |
| Gene ID: | 7137 |
| Uniprot: | P19429 |
| Cellular Location: | |
| Calculated MW: | 24kDa |
| Observed MW: | 26kDa |
| Synonyms: | TNNI3, CMD1FF, CMD2A, CMH7, RCM1, TNNC1, cTnI, troponin I3 |
| Background: | Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). |
| UniProt Protein Function: | TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family. |
| UniProt Protein Details: | Protein type:Actin-binding; Motility/polarity/chemotaxis; Motor Chromosomal Location of Human Ortholog: 19q13.4 Cellular Component: cytosol; sarcomere; troponin complex Molecular Function:actin binding; calcium channel inhibitor activity; calcium-dependent protein binding; metal ion binding; protein binding; protein domain specific binding; protein kinase binding; troponin C binding; troponin T binding Biological Process: cardiac muscle contraction; cellular calcium ion homeostasis; heart contraction; heart development; muscle filament sliding; negative regulation of ATPase activity; regulation of smooth muscle contraction; regulation of systemic arterial blood pressure by ischemic conditions; skeletal muscle contraction; vasculogenesis; ventricular cardiac muscle morphogenesis Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Dilated, 2a; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Familial Restrictive, 1 |
| NCBI Summary: | Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008] |
| UniProt Code: | P19429 |
| NCBI GenInfo Identifier: | 136213 |
| NCBI Gene ID: | 7137 |
| NCBI Accession: | P19429.3 |
| UniProt Secondary Accession: | P19429,P48787, P23693, |
| UniProt Related Accession: | P19429 |
| Molecular Weight: | 24,008 Da |
| NCBI Full Name: | Troponin I, cardiac muscle |
| NCBI Synonym Full Names: | troponin I3, cardiac type |
| NCBI Official Symbol: | TNNI3Â Â |
| NCBI Official Synonym Symbols: | CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FFÂ Â |
| NCBI Protein Information: | troponin I, cardiac muscle |
| UniProt Protein Name: | Troponin I, cardiac muscle |
| UniProt Synonym Protein Names: | Cardiac troponin I |
| Protein Family: | Troponin |
| UniProt Gene Name: | TNNI3Â Â |
| UniProt Entry Name: | TNNI3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TNNI3 antibody (CAB6995) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of mouse heart cells using TNNI3 antibody (CAB6995) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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