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Total Antioxidant Capacity Assay Kit - Colorimetric (MAES0168)

SKU:
MAES0168
Product Type:
Assay
Detection Method:
Colorimetric
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma, Urine, Saliva, Tissue, Cells
Sensitivity:
0.05 mmol/L
Range:
0.05-1.00 mmol/L
Assay Time:
20 min
Shipping:
Gel Pack
Research Area:
Oxidative Stress
Antioxidant
Plant & Environmental Stress
€299
Frequently bought together:

Description

system_update_altDatasheet
Product Name:Total Antioxidant Capacity Assay Kit - Colorimetric
Product Code:MAES0168
Product Size:96 Assays
Sample Type:Serum, plasma, urine, saliva, tissue, cells
Assay Time:20 min
Instrument:Microplate reader
Sensitivity:0.05 mmol/L
Detection Range:0.05-1.00 mmol/L
Recovery Rate:102
Inter CV:5
Inter CV:4.1

There are two kinds of antioxidant system, one is enzyme antioxidant system, including superoxide dismutase (SOD),) catalase (CAT), glutathione peroxidase (GSH-Px). The other is non-enzymatic antioxidant systems, including uric acid, vitamin C, vitamin E, glutathione, bilirubin, α-lipoic acid, carotenoid. Antioxidant capacity is thought to be the cumulative effect of all antioxidants in blood and body fluids.

The principle of the ABTS method for determining the T-AOC is as follows. ABTS is oxidized to green ABTS+ by appropriate oxidant, which can be inhibited if there exist antioxidants. The T-AOC of the sample can be determined and calculated by measuring the absorbance of ABTS+ at 734 nm. Trolox is an analog of VE and has a similar antioxidant capacity to that of VE. Trolox is used as a reference for other antioxidant antioxidants. For example, the T-AOC of Trolox is 1, then the antioxidant capacity of the other substance with the same concentration is showed by the ratio of its antioxidant capacity to Trolox antioxidant capacity.

Shelf Life:6 Months
Storage:-20°C
Shipping:Gel Pack
Research Area:Oxidative stress

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