Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (ABTS, Chemical) (MAES0168)
- SKU:
- MAES0168
- Product Type:
- Assay
- Detection Method:
- Colorimetric
- Instrument:
- Microplate Reader
- Sample Type:
- Serum, Plasma, Urine, Saliva, Tissue, Cells
- Sensitivity:
- 0.05 mmol/L
- Range:
- 0.05-1.00 mmol/L
- Assay Time:
- 20 min
- Shipping:
- Gel Pack
- Research Area:
- Oxidative Stress
- Antioxidant
- Plant & Environmental Stress
Description
| Product Name: | Total Antioxidant Capacity Assay Kit - Colorimetric |
| Product Code: | MAES0168 |
| Product Size: | 96 Assays |
| Sample Type: | Serum, plasma, urine, saliva, tissue, cells |
| Assay Time: | 20 min |
| Instrument: | Microplate reader |
| Sensitivity: | 0.05 mmol/L |
| Detection Range: | 0.05-1.00 mmol/L |
| Recovery Rate: | 102 |
| Inter CV: | 5 |
| Inter CV: | 4.1 |
There are two kinds of antioxidant system, one is enzyme antioxidant system, including superoxide dismutase (SOD),) catalase (CAT), glutathione peroxidase (GSH-Px). The other is non-enzymatic antioxidant systems, including uric acid, vitamin C, vitamin E, glutathione, bilirubin, α-lipoic acid, carotenoid. Antioxidant capacity is thought to be the cumulative effect of all antioxidants in blood and body fluids.
The principle of the ABTS method for determining the T-AOC is as follows. ABTS is oxidized to green ABTS+ by appropriate oxidant, which can be inhibited if there exist antioxidants. The T-AOC of the sample can be determined and calculated by measuring the absorbance of ABTS+ at 734 nm. Trolox is an analog of VE and has a similar antioxidant capacity to that of VE. Trolox is used as a reference for other antioxidant antioxidants. For example, the T-AOC of Trolox is 1, then the antioxidant capacity of the other substance with the same concentration is showed by the ratio of its antioxidant capacity to Trolox antioxidant capacity.
| Shelf Life: | 6 Months |
| Storage: | -20°C |
| Shipping: | Gel Pack |
| Research Area: | Oxidative stress |
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