Total Sulfhydryl Group/Total Thiol (-SH) Assay Kit - Colorimetric (MAES0167)
- SKU:
- MAES0167
- Product Type:
- Assay
- Detection Method:
- Colorimetric
- Instrument:
- Microplate Reader
- Sample Type:
- Serum, Plasma, Animal Tissue
- Sensitivity:
- 9.91 umol/L
- Range:
- 9.91-1000 umol/L
- Assay Time:
- 20 min
- Shipping:
- Gel Pack
- Research Area:
- Oxidative Stress
- Antioxidant
Description
| Product Name: | Total Sulfhydryl Group/Total Thiol (-SH) Assay Kit - Colorimetric |
| Product Code: | MAES0167 |
| Product Size: | 96 Assays |
| Sample Type: | Serum, plasma, animal tissue |
| Assay Time: | 20 min |
| Instrument: | Microplate reader |
| Sensitivity: | 9.91 µmol/L |
| Detection Range: | 9.91-1000 µmol/L |
| Recovery Rate: | 104 |
| Inter CV: | 2.9 |
| Inter CV: | 2.5 |
Total sulfhydryl group/total thiol (-SH) is an important protein structure and REDOX reactive group in organisms. Sulfhydryl is one of the most active and ubiquitous ligands in biological systems. It is found in most proteins, but also in some low molecular weight substances. Sulfhydryl groups play an important role in biochemical processes, not only for the REDOX mechanism, but also for the enhancement of the function of some hydrolyzed biocatalysts. Compounds containing sulfhydryl groups are called thiols. Decreased thiol levels are found in various diseases.
Sulfhydryl compounds react with 5,5' -dithiobis (2-nitrobenzoic acid) under neutral or alkaline conditions to produce a yellow product which have a maximum absorption peak at 412 nm. Measure the OD value and calculate the total mercapto content indirectly.
| Shelf Life: | 6 Months |
| Storage: | 2-8°C |
| Shipping: | Gel Pack |
| Research Area: | Oxidative stress |
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