Description
| Product Name: | Total Superoxide Dismutase Activity Assay Kit |
| Product Code: | MAES0034 |
| Product Size: | 96 Assays |
| Sample Type: | Serum, plasma, hydrothorax, ascites, urine, cells, tissue |
| Assay Time: | 30 min |
| Instrument: | Microplate reader |
| Sensitivity: | 0.2 U/mL |
| Detection Range: | 0.2 -14.4 U/mL |
| Recovery Rate: | 97 |
| Inter CV: | 3.7 |
| Inter CV: | 2.9 |
According to the literature, superoxide dismutase exists in all oxygen-metabolizing cells to protect cells from excessive superoxide. Under the action of SOD, two superoxide anions were converted to oxygen and hydrogen peroxide. The reaction principle is as follows:In mammals, there are three different forms of SOD: CuZn-SOD, Mn-SOD and EC-SOD (an extracellular form of SOD). Cu-Zn SOD exists in the cytoplasmic and mitochondrial membrane spaces of the cells, while Mn-SOD is located in the mitochondrial matrix.
The activity of SOD was measured by WST-1 method in this kit and the principles of the WST-1 is as follows. Xanthine Oxidase (XO) can catalyze WST-1 react with O2- to generate a water-soluble formazan dye. SOD can catalyze the disproportionation of superoxide anions, so the reaction can be inhibited by SOD, and the activity of SOD is negatively correlated with the amount of formazan dye. Therefore, the activity of SOD can be determined by the colorimetric analysis of WST-1 products.
| Shelf Life: | 12 Months |
| Storage: | Reagent 3:-20°C;, Others:2-8°C |
| Shipping: | Gel Pack |
| Research Area: | Enzymes, Oxidative stress |
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