Description
Tovetumab ELISA Kit
The Tovetumab ELISA Kit (SKU: GXEL00144) is a quantitative sandwich ELISA designed for the accurate quantitative detection of Tovetumab across Plasma, Serum. With a working range of 0.31-5 μg/mL and a sensitivity of 0.156 μg/ml, the kit combines a simple protocol with dependable colorimetric read-out for confident quantification.
Tovetumab (MEDI-575) is a fully human IgG2κ monoclonal antibody that specifically binds to human platelet-derived growth factor receptor alpha (PDGFRα) and blocks receptor signal transduction by PDGF ligands. The affinity of tovetumab determined using surface plasmon resonance technology and flow cytometry demonstrated comparable binding affinity for human and monkey PDGFRα. In single and repeat-dose monkey pharmacokinetic-pharmacodynamic (PK-PD) studies, tovetumab administration resulted in dose-dependent elevation of circulating levels of PDGF-AA, a member of the PDGF ligand family, due to displacement of PDGF-AA from PDGFRα by tovetumab and subsequent blockade of PDGFRα-mediated PDGF-AA degradation. As such, PDGF-AA accumulation is an indirect measurement of receptor occupancy and is a novel PD biomarker for tovetumab. The nonlinear PK of tovetumab and dose-dependent increase in circulating PDGF-AA profiles were well described by a novel mechanistic model, in which tovetumab and PDGF-AA compete for the binding to PDGFRα.
Backed by Assay Genie quality control and technical support, this Tovetumab ELISA Kit is an ideal tool for laboratories studying Tovetumab. Order the Tovetumab ELISA Kit from Assay Genie for dependable performance.
| Product Name: | Tovetumab ELISA Kit |
| SKU: | GXEL00144 |
| Accession: | P16234 |
| Alternative Names: | MEDI-575, 1243266-04-7 |
| Assay Type: | Quantitative |
| Detection Method: | Colorimetric |
| Sensitivity: | 0.156 μg/ml |
| Range: | 0.31-5 μg/mL |
| Sample Type: | Plasma, Serum |
| Storage: | 2-8°C. See Storage & Stability for details. |
| Note: | For Research Use Only. Not for diagnostic procedures. |
| Parameter | Specification |
|---|---|
| Assay Type | Quantitative |
| Detection Method | Colorimetric |
| Sensitivity | 0.156 μg/ml |
| Detection Range | 0.31-5 μg/mL |
| Recovery | 80-120% |
| Sample Types | Plasma, Serum |
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% prior to the expiration date under appropriate storage condition.
Store the kit at 2-8°C on receipt. Avoid repeated freeze-thaw cycles.
