The [KO Validated] p53 Antibody (CAB5761) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits strong reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the TP53 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for investigations in oncology and cancer biology.TP53, also known as p53, is a key player in regulating cell cycle progression and apoptosis, serving as a guardian of the genome by preventing the growth of abnormal cells that could lead to cancer development.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
[KO Validated] p53 Antibody
SKU:
CAB5761
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Purification Method
Affinity purification
Gene ID
7157
RRID
AB_2766515
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Immunoprecipitation analysis of 200 μg extracts of U-251MG cells using 3 μg p53 antibody (CAB5761). Western blot was performed from the immunoprecipitate using p53 antibody (CAB5761) at a dilution of 1:1000.
Immunoprecipitation analysis of 200 μg extracts of U-251MG cells using 3 μg p53 antibody (CAB5761). Western blot was performed from the immunoprecipitate using p53 antibody (CAB5761) at a dilution of 1:1000.
