Anti-TPH2 Antibody (CAB7147)

Antibody Name:	Anti-TPH2 Antibody
Antibody SKU:	CAB7147
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human TPH2 (NP_775489.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse skeletal muscle, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human TPH2 (NP_775489.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MQPA MMMF SSKY WARR GFSL DSAV PEEH QLLG SSTL NKPN SGKN DDKG NKGS SKRE AATE SGKT AVVF SLKN EVGG LVKA LRLF QEKR VNMV HIES RKSR RRSS EVEI FVDC ECGK TEFN ELIQ LLKF QTTI VTLN PPEN IWTE EEEL EDVP WFPR KISE LDKC SHRV LM
Gene ID:	121278
Uniprot:	Q8IWU9
Cellular Location:
Calculated MW:	56kDa
Observed MW:	56kDa

Synonyms:	TPH2, ADHD7, NTPH
Background:	This gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression.

UniProt Protein Function:	TPH2: an enzyme that is rate-limiting in the biosynthesis of serotonin. It catalyzes the biopterin dependent monooxygenation of tryptophan to 5-hydroxytryptophan (5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. Its expression is limited to a few specialized tissues: raphe neurons, pinealocytes, mast cells, mononuclear leukocytes, beta-cells of the islets of Langerhans, and intestinal and pancreatic enterochromaffin cells. Two alternatively spliced isoforms have been described. Isoform 2 appears to be expressed exclusively in the brain, and is expressed at significantly higher levels in the raphe nuclei than isoform 1.
UniProt Protein Details:	Protein type:Oxidoreductase; EC 1.14.16.4; Amino Acid Metabolism - tryptophan Chromosomal Location of Human Ortholog: 12q21.1 Cellular Component: cytosol Biological Process: indolalkylamine biosynthetic process Disease: Attention Deficit-hyperactivity Disorder, Susceptibility To, 7; Major Depressive Disorder
NCBI Summary:	This gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression. [provided by RefSeq, Feb 2016]
UniProt Code:	Q8IWU9
NCBI GenInfo Identifier:	30580625
NCBI Gene ID:	121278
NCBI Accession:	Q8IWU9.1
UniProt Secondary Accession:	Q8IWU9,Q14CB0, A6NGA4,
UniProt Related Accession:	Q8IWU9
Molecular Weight:	56,242 Da
NCBI Full Name:	Tryptophan 5-hydroxylase 2
NCBI Synonym Full Names:	tryptophan hydroxylase 2
NCBI Official Symbol:	TPH2
NCBI Official Synonym Symbols:	NTPH; ADHD7
NCBI Protein Information:	tryptophan 5-hydroxylase 2
UniProt Protein Name:	Tryptophan 5-hydroxylase 2
UniProt Synonym Protein Names:	Neuronal tryptophan hydroxylase; Tryptophan 5-monooxygenase 2
Protein Family:	Tryptophan 5-hydroxylase
UniProt Gene Name:	TPH2
UniProt Entry Name:	TPH2_HUMAN

	Western blot analysis of extracts of various cell lines, using TPH2 antibody (CAB7147) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse brain using TPH2 antibody (CAB7147) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of A549 cells using TPH2 antibody (CAB7147). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-TPH2 Antibody (CAB14520)	Human TPH2 ELISA Kit
Phospho-TPH2 (S19) Antibody (PACO02449)	Human Tryptophan 5-hydroxylase 2 (TPH2) ELISA Kit
TPH2 Antibody (PACO02450)	TPH2 Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)