Anti-TPM3 Antibody (CAB13557)

Antibody Name:	Anti-TPM3 Antibody
Antibody SKU:	CAB13557
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-284 of human TPM3 (NP_689476.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Mouse
Positive Samples:	Mouse skeletal muscle, Mouse lung, Mouse heart, Mouse spinal cord

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-284 of human TPM3 (NP_689476.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MMEA IKKK MQML KLDK ENAL DRAE QAEA EQKQ AEER SKQL EDEL AAMQ KKLK GTED ELDK YSEA LKDA QEKL ELAE KKAA DAEA EVAS LNRR IQLV EEEL DRAQ ERLA TALQ KLEE AEKA ADES ERGM KVIE NRAL KDEE KMEL QEIQ LKEA KHIA EEAD RKYE EVAR KLVI IEGD LERT EERA ELAE SKCS ELEE ELKN VTNN LKSL EAQA EKYS QKED KYEE EIKI LTDK LKEA ETRA EFAE RSVA KLEK TIDD LEDE LYAQ KLKY KAIS EELD HALN DMTS
Gene ID:	7170
Uniprot:	P06753
Cellular Location:	Cytoplasm, cytoskeleton
Calculated MW:	18kDa/28kDa/29kDa/32kDa
Observed MW:	37kDa

Synonyms:

TPM3, CAPM1, CFTD, HEL-189, HEL-S-82p, NEM1, OK/SW-cl.5, TM-5, TM3, TM30, TM30nm, TM5, TPMsk3, TRK, hscp30

Background:

This gene encodes a member of the tropomyosin family of actin-binding proteins. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. Mutations in this gene result in autosomal dominant nemaline myopathy and other muscle disorders. This locus is involved in translocations with other loci, including anaplastic lymphoma receptor tyrosine kinase (ALK) and neurotrophic tyrosine kinase receptor type 1 (NTRK1), which result in the formation of fusion proteins that act as oncogenes. There are numerous pseudogenes for this gene on different chromosomes. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	TPM3: a cytoskeletal protein that binds to actin filaments in muscle and nonmuscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Defects in TPM3 are a cause of nemaline myopathy type 1 (NEM1). Three alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Contractile; Cytoskeletal; Oncoprotein; Actin-binding; Motor; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1q21.2 Cellular Component: cortical cytoskeleton; filamentous actin; cytoskeleton; growth cone; stress fiber; podosome; cytosol; muscle thin filament tropomyosin; cleavage furrow Molecular Function:actin binding Biological Process: muscle contraction; cell motility; muscle filament sliding Disease: Nemaline Myopathy 1; Myopathy, Congenital, With Fiber-type Disproportion
NCBI Summary:	This gene encodes a member of the tropomyosin family of actin-binding proteins. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. Mutations in this gene result in autosomal dominant nemaline myopathy and other muscle disorders. This locus is involved in translocations with other loci, including anaplastic lymphoma receptor tyrosine kinase (ALK) and neurotrophic tyrosine kinase receptor type 1 (NTRK1), which result in the formation of fusion proteins that act as oncogenes. There are numerous pseudogenes for this gene on different chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]
UniProt Code:	P06753
NCBI GenInfo Identifier:	519668659
NCBI Gene ID:	7170
NCBI Accession:	P06753.2
UniProt Secondary Accession:	P06753,P12324, Q2QD06, Q5VU58, Q5VU63, Q5VU66, Q5VU71 Q5VU72, Q8TCG3, Q969Q2, Q9NQH8, D3DV71,
UniProt Related Accession:	P06753
Molecular Weight:
NCBI Full Name:	Tropomyosin alpha-3 chain
NCBI Synonym Full Names:	tropomyosin 3
NCBI Official Symbol:	TPM3
NCBI Official Synonym Symbols:	TM3; TM5; TRK; CFTD; NEM1; TM-5; TM30; CAPM1; TM30nm; TPMsk3; hscp30; HEL-189; HEL-S-82p; OK/SW-cl.5
NCBI Protein Information:	tropomyosin alpha-3 chain; tropomyosin-5; tropomyosin gamma; cytoskeletal tropomyosin TM30; epididymis luminal protein 189; alpha-tropomyosin, slow skeletal; heat-stable cytoskeletal protein 30 kDa; epididymis secretory sperm binding protein Li 82p
UniProt Protein Name:	Tropomyosin alpha-3 chain
UniProt Synonym Protein Names:	Gamma-tropomyosin; Tropomyosin-3; Tropomyosin-5; hTM5
Protein Family:	Tropomyosin
UniProt Gene Name:	TPM3
UniProt Entry Name:	TPM3_HUMAN

	Western blot analysis of extracts of various cell lines, using TPM3 antibody (CAB13557) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded mouse heart using TPM3 antibody (CAB13557) at dilution of 1:100 (40x lens).

Antibodies

Anti-TPM3 Antibody (CAB1206)

TPM3 Antibody (PACO12865)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)