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Anti-TRAF7 Antibody (CAB15607)

SKU:
CAB15607
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
€139 - €419
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Description

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Antibody Name:Anti-TRAF7 Antibody
Antibody SKU:CAB15607
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 260-400 of human TRAF7 (NP_115647.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 260-400 of human TRAF7 (NP_115647.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PHSK YGCT FIGN QDTY ETHL ETCR FEGL KEFL QQTD DRFH EMHV ALAQ KDQE IAFL RSML GKLS EKID QLEK SLEL KFDV LDEN QSKL SEDL MEFR RDAS MLND ELSH INAR LNMG ILGS YDPQ QIFK CKGT FVGH QGPV W
Gene ID:84231
Uniprot:Q6Q0C0
Cellular Location:
Calculated MW:66kDa/74kDa
Observed MW:75kDa
Synonyms:TRAF7, RFWD1, RNF119
Background:Tumor necrosis factor (TNF; see MIM 191160) receptor-associated factors, such as TRAF7, are signal transducers for members of the TNF receptor superfamily (see MIM 191190). TRAFs are composed of an N-terminal cysteine/histidine-rich region containing zinc RING and/or zinc finger motifs; a coiled-coil (leucine zipper) motif; and a homologous region that defines the TRAF family, the TRAF domain, which is involved in self-association and receptor binding.
UniProt Protein Function:TRAF7: E3 ubiquitin ligase capable of auto-ubiquitination, following phosphorylation by MAP3K3. Potentiates MEKK3-mediated activation of the NF-kappa-B, JUN/AP1 and DDIT3 transcriptional regulators. Induces apoptosis when overexpressed. Belongs to the WD repeat TRAF7 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system; EC 6.3.2.-; Ubiquitin ligase; EC 6.3.2.19; Ligase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasmic membrane-bound vesicle; plasma membrane; nucleus; cytosol; ubiquitin ligase complex

Molecular Function:protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: positive regulation of protein sumoylation; regulation of protein localization; positive regulation of MAPKKK cascade; transcription, DNA-dependent; apoptosis; activation of MAPKKK activity; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter

NCBI Summary:Tumor necrosis factor (TNF; see MIM 191160) receptor-associated factors, such as TRAF7, are signal transducers for members of the TNF receptor superfamily (see MIM 191190). TRAFs are composed of an N-terminal cysteine/histidine-rich region containing zinc RING and/or zinc finger motifs; a coiled-coil (leucine zipper) motif; and a homologous region that defines the TRAF family, the TRAF domain, which is involved in self-association and receptor binding.[supplied by OMIM, Apr 2004]
UniProt Code:Q6Q0C0
NCBI GenInfo Identifier:54036486
NCBI Gene ID:84231
NCBI Accession:Q6Q0C0.1
UniProt Secondary Accession:Q6Q0C0,Q9H073,
UniProt Related Accession:Q6Q0C0
Molecular Weight:670
NCBI Full Name:E3 ubiquitin-protein ligase TRAF7
NCBI Synonym Full Names:TNF receptor-associated factor 7, E3 ubiquitin protein ligase
NCBI Official Symbol:TRAF7  
NCBI Official Synonym Symbols:RFWD1; RNF119  
NCBI Protein Information:E3 ubiquitin-protein ligase TRAF7; RING finger protein 119; ring finger and WD repeat domain 1; RING finger and WD repeat-containing protein 1
UniProt Protein Name:E3 ubiquitin-protein ligase TRAF7
UniProt Synonym Protein Names:RING finger and WD repeat-containing protein 1; RING finger protein 119; TNF receptor-associated factor 7
Protein Family:E3 ubiquitin-protein ligase
UniProt Gene Name:TRAF7  
UniProt Entry Name:TRAF7_HUMAN
Anti-TRAF7 Antibody (CAB15607)Western blot analysis of extracts of various cell lines, using TRAF7 antibody (CAB15607) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.

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