Description
TRIM38 Colorimetric Cell-Based ELISA
The TRIM38 Colorimetric Cell-Based ELISA Kit is a cutting-edge tool for quantifying TRIM38 protein levels in cell lysates and tissue homogenates. With its high sensitivity and specificity, this kit provides accurate and reliable results, making it ideal for a variety of research applications.TRIM38 is a member of the tripartite motif (TRIM) family of proteins, known for their roles in regulating various cellular processes such as immune response, cell signaling, and viral defense mechanisms. TRIM38 specifically plays a crucial role in inflammation, autophagy, and innate immune response pathways, making it a key player in understanding the mechanisms underlying these processes.
By using the TRIM38 Colorimetric Cell-Based ELISA Kit, researchers can gain valuable insights into the function and regulation of TRIM38 in cellular pathways and disease states. This kit is an essential tool for studying inflammation, autophagy, and immune response-related disorders, as well as for developing potential therapeutics targeting TRIM38.
| Product Name: | TRIM38 Colorimetric Cell-Based ELISA |
| Product Code: | CBCAB00922 |
| ELISA Type: | Cell-Based |
| Target: | TRIM38 |
| Reactivity: | Human, Mouse |
| Dynamic Range: | > 5000 Cells |
| Detection Method: | Colorimetric 450 nmStorage/Stability:4°C/6 Months |
| Format: | 96-Well Microplate |
The TRIM38 Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect TRIM38 protein expression profile in cells. The kit can be used for measuring the relative amounts of TRIM38 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on TRIM38.
Qualitative determination of TRIM38 concentration is achieved by an indirect ELISA format. In essence, TRIM38 is captured by TRIM38-specific primary antibodies while the HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the HRP enzyme conjugated to the secondary antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:
| 1. | A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. |
| 2. | Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. |
| Database Information: | Gene ID: 10475, UniProt ID: O00635, OMIM: 0, Unigene: Hs.584851 |
| Gene Symbol: | TRIM38 |
| Sub Type: | None |
| UniProt Protein Function: | TRIM38: E3 ubiquitin-protein ligase. Mediates 'Lys-48'-linked polyubiquitination and proteasomal degradation of the critical TLR adapter TICAM1, inhibiting TLR3-mediated type I interferon signaling. {ECO:0000269|PubMed:23056470} |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: cytosol Molecular Function:protein binding; signal transducer activity Biological Process: activation of NF-kappaB transcription factor; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of transcription factor activity; positive regulation of virion penetration into host cell |
| NCBI Summary: | This gene encodes a member of the tripartite motif (TRIM) family. The encoded protein contains a RING-type zinc finger, B box-type zinc finger and SPRY domain. The function of this protein has not been identified. A pseudogene of this gene is located on the long arm of chromosome 4. [provided by RefSeq, Jul 2012] |
| UniProt Code: | O00635 |
| NCBI GenInfo Identifier: | 21362879 |
| NCBI Gene ID: | 10475 |
| NCBI Accession: | O00635.1 |
| UniProt Secondary Accession: | O00635,B2R862, |
| UniProt Related Accession: | O00635 |
| Molecular Weight: | 53,416 Da |
| NCBI Full Name: | E3 ubiquitin-protein ligase TRIM38 |
| NCBI Synonym Full Names: | tripartite motif containing 38 |
| NCBI Official Symbol: | TRIM38 |
| NCBI Official Synonym Symbols: | RNF15; RORET |
| NCBI Protein Information: | E3 ubiquitin-protein ligase TRIM38 |
| UniProt Protein Name: | E3 ubiquitin-protein ligase TRIM38 |
| UniProt Synonym Protein Names: | RING finger protein 15; Tripartite motif-containing protein 38; Zinc finger protein RoRet |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | TRIM38 |
| UniProt Entry Name: | TRI38_HUMAN |
| Component | Quantity |
| 96-Well Cell Culture Clear-Bottom Microplate | 2 plates |
| 10X TBS | 24 mL |
| Quenching Buffer | 24 mL |
| Blocking Buffer | 50 mL |
| 15X Wash Buffer | 50 mL |
| Primary Antibody Diluent | 12 mL |
| 100x Anti-Phospho Target Antibody | 60 µL |
| 100x Anti-Target Antibody | 60 µL |
| Anti-GAPDH Antibody | 60 µL |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12 mL |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12 mL |
| SDS Solution | 12 mL |
| Stop Solution | 24 mL |
| Ready-to-Use Substrate | 12 mL |
| Crystal Violet Solution | 12 mL |
| Adhesive Plate Seals | 2 seals |
The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing ≥1 ml
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
- Orbital shaker (optional)
- Deionized or sterile water
*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1. | Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37°C prior to adding cells. |
| 2. | Incubate the cells for overnight at 37°C, 5% CO2. |
| 3. | Treat the cells as desired. |
| 4. | Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice. |
| 5. | Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells. |
| 6. | Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4°C for a week. |
| 7. | Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature. |
| 8. | Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time. |
| 9. | Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature. |
| 10. | Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time. |
| 11. | Add 50 µL of 1x primary antibodies (Anti-TRIM38 Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4°C. If the target expression is known to be high, incubate for 2 hours at room temperature. |
| 12. | Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time. |
| 13. | Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature. |
| 14. | Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time. |
| 15. | Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark. |
| 16. | Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader. |
(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)
