Anti-TRMT2A Antibody (CAB7095)

Antibody Name:	Anti-TRMT2A Antibody
Antibody SKU:	CAB7095
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human TRMT2A (NP_073564.3).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human
Positive Samples:	HL-60, Raji, NCI-H460

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human TRMT2A (NP_073564.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSEN LDNE GPKP MESC GQES SSAL SCPT VSVP PAAP AALE EVEK EGAG AATG PGPQ PGLY SYIR DDLF TSEI FKLE LQNV PRHA SFSD VRRF LGRF GLQP HKTK LFGQ PPCA FVTF RSAA ERDK ALRV LHGA LWKG RPLS VRLA RPKA DPMA RRRR QEGE SEPP VTRV ADVV TPLW TVPY AEQL ERKQ LECE QVLQ KLAK EIGS TNRA LLPW LLEQ RHKH NKAC CPLE GVRP SPQQ TEYR NKCE FLVG VG
Gene ID:	27037
Uniprot:	Q8IZ69
Cellular Location:
Calculated MW:	61kDa/68kDa
Observed MW:	75kDa

Synonyms:	TRMT2A, HTF9C
Background:	The protein encoded by this gene is of unknown function. However, it is orthologous to the mouse Trmt2a gene and contains an RNA methyltransferase domain. Expression of this gene varies during the cell cycle, with aberrant expression being a possible biomarker in certain breast cancers. Several transcript variants encoding two different isoforms have been found for this gene.

UniProt Protein Function:	HTF9C: May be involved in nucleic acid metabolism and/or modifications. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 22q11.21 Molecular Function:nucleotide binding; RNA methyltransferase activity Biological Process: RNA processing; RNA methylation
NCBI Summary:	The protein encoded by this gene is of unknown function. However, it is orthologous to the mouse Trmt2a gene and contains an RNA methyltransferase domain. Expression of this gene varies during the cell cycle, with aberrant expression being a possible biomarker in certain breast cancers. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
UniProt Code:	Q8IZ69
NCBI GenInfo Identifier:	51316479
NCBI Gene ID:	27037
NCBI Accession:	Q8IZ69.2
UniProt Secondary Accession:	Q8IZ69,Q32P57, Q96ME6, Q9H732, D3DX25,
UniProt Related Accession:	Q8IZ69
Molecular Weight:	625
NCBI Full Name:	tRNA (uracil-5-)-methyltransferase homolog A
NCBI Synonym Full Names:	tRNA methyltransferase 2 homolog A (S. cerevisiae)
NCBI Official Symbol:	TRMT2A
NCBI Official Synonym Symbols:	HTF9C
NCBI Protein Information:	tRNA (uracil-5-)-methyltransferase homolog A; tRNA (uracil-5-)-methyltransferase homolog A; hpaII tiny fragments locus 9c protein; TRM2 tRNA methyltransferase 2 homolog A
UniProt Protein Name:	tRNA (uracil-5-)-methyltransferase homolog A
UniProt Synonym Protein Names:	HpaII tiny fragments locus 9c protein
Protein Family:	tRNA
UniProt Gene Name:	TRMT2A
UniProt Entry Name:	TRM2A_HUMAN

	Western blot analysis of extracts of various cell lines, using TRMT2A antibody (CAB7095) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human lung cancer using TRMT2A antibody (CAB7095) at dilution of 1:100 (40x lens).

Antibodies

Anti-TRMT2A Antibody (CAB5975)

Anti-TRMT2A Antibody (CAB19929)[KO Validated]

Anti-TRMT2A Antibody (CAB19987)[KO Validated]

TRMT2A Antibody (PACO12931)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)