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Anti-TSFM Antibody (CAB16094)

SKU:
CAB16094
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
  • Metabolism Antibodies 3 Anti-TSFM Antibody CAB16094
  • Metabolism Antibodies 3 Anti-TSFM Antibody CAB16094
$167 - $503
Frequently bought together:

Description

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Antibody Name:Anti-TSFM Antibody
Antibody SKU:CAB16094
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 245-346 of human TSFM (NP_001166167).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, HeLa, Jurkat, U-251MG, Mouse heart, Mouse liver, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 245-346 of human TSFM (NP_001166167).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PSLH KLVL GKYG ALVI CETS EQKT NLED VGRR LGQH VVGM APLS VGSL DDEP GGEA ETKM LSQP YLLD PSIT LGQY VQPQ GVSV VDFV RFEC GEGE EAAE TE
Gene ID:10102
Uniprot:P43897
Cellular Location:Mitochondrion
Calculated MW:18kDa/23kDa/35kDa/37kDa
Observed MW:35kDa
Synonyms:TSFM, EFTS, EFTSMT
Background:This gene encodes a mitochondrial translation elongation factor. The encoded protein is an enzyme that catalyzes the exchange of guanine nucleotides on the translation elongation factor Tu during the elongation step of mitchondrial protein translation. Mutations in this gene are associated with combined oxidative phosphorylation deficiency-3 syndrome. Alternate splicing results in multiple transcript variants.
UniProt Protein Function:EFT5: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome. Defects in TSFM are the cause of combined oxidative phosphorylation deficiency type 3 (COXPD3). Defects in the mitochondrial oxidative phosphorylation system result in devastating, mainly multisystem, diseases. COXPD3 symptoms include severe metabolic acidosis with encephalomyopathy or with hypertrophic cardiomyopathy. Patients show a severe defect in mitochondrial translation leading to a failure to assemble adequate amounts of three of the oxidative phosphorylation complexes. Belongs to the EF-Ts family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Translation elongation; Translation

Chromosomal Location of Human Ortholog: 12q14.1

Cellular Component: mitochondrial matrix; mitochondrion; nucleus

Molecular Function:translation elongation factor activity

Biological Process: regulation of RNA elongation; translational elongation

Disease: Combined Oxidative Phosphorylation Deficiency 3

NCBI Summary:This gene encodes a mitochondrial translation elongation factor. The encoded protein is an enzyme that catalyzes the exchange of guanine nucleotides on the translation elongation factor Tu during the elongation step of mitchondrial protein translation. Mutations in this gene are associated with combined oxidative phosphorylation deficiency-3 syndrome. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Mar 2010]
UniProt Code:P43897
NCBI GenInfo Identifier:12644268
NCBI Gene ID:10102
NCBI Accession:P43897.2
UniProt Secondary Accession:P43897,Q561V7, Q8TBC2, Q9UQK0, B4E391, F5H2T7,
UniProt Related Accession:P43897
Molecular Weight:23,557 Da
NCBI Full Name:Elongation factor Ts, mitochondrial
NCBI Synonym Full Names:Ts translation elongation factor, mitochondrial
NCBI Official Symbol:TSFM  
NCBI Official Synonym Symbols:EFTS; EFTSMT  
NCBI Protein Information:elongation factor Ts, mitochondrial
UniProt Protein Name:Elongation factor Ts, mitochondrial
UniProt Gene Name:TSFM  
UniProt Entry Name:EFTS_HUMAN
Anti-TSFM Antibody (CAB16094)Western blot analysis of extracts of various cell lines, using TSFM antibody (CAB16094) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-TSFM Antibody (CAB16094)Immunofluorescence analysis of U-2 OS cells using TSFM Polyclonal Antibody (CAB16094) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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