The UBE2E1 Antibody (CAB13912) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high specificity and sensitivity in detecting UBE2E1 in human samples, making it ideal for use in Western blotting and immunohistochemistry applications.UBE2E1 is a crucial component of the ubiquitin-proteasome system, responsible for targeting proteins for degradation and regulating various cellular processes, including cell cycle progression, DNA repair, and protein quality control. Dysregulation of UBE2E1 has been implicated in various diseases, such as cancer, neurodegenerative disorders, and autoimmune conditions, making it a promising therapeutic target for drug development and disease intervention strategies.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
UBE2E1 Antibody
SKU:
CAB13912
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
UBCH6, UBE2E1
Positive Sample:
HT-29, HeLa, K-562
Cellular Localization:
Nucleus.
Calculated MW:
21kDa
Observed MW:
21kDa
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
7324
RRID
AB_2772775
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using UBE2E1 Rabbit pAb (CAB13912) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
