Western blot analysis of extracts of HeLa cells, using UCHL5IP antibody (CAB21156) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The UCHL5IP Monoclonal Antibody (CAB21156) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated by mouse hybridoma technology, is highly specific for human UCHL5IP and has been validated for use in applications such as Western blot and immunohistochemistry.UCHL5IP, also known as ubiquitin C-terminal hydrolase L5 interacting protein, plays a critical role in the regulation of protein degradation and turnover. By targeting UCHL5IP, researchers can gain insights into the mechanisms underlying protein homeostasis and potentially identify new therapeutic targets for diseases related to protein misfolding or aggregation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
UCHL5IP Monoclonal Antibody
SKU:
CAB21156
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC3021
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
UIP1, UCHL5IP
Positive Sample:
HeLa
Cellular Localization:
Centrosome, Cytosol, Nucleolus, Plasma Membrane.
Calculated MW:
40kDa
Observed MW:
40kDa
This gene encodes a subunit of the augmin complex, which regulates centrosome and mitotic spindle integrity, and is necessary for the completion of cytokinesis. The encoded protein was identified by interaction with ubiquitin C-terminal hydrolase 37. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
55559
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using UCHL5IP Rabbit mAb (CAB21156) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 120s.
