Anti-UCP3 Antibody (CAB16995)

Antibody Name:	Anti-UCP3 Antibody
Antibody SKU:	CAB16995
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human UCP3.

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	RD, Mouse heart, Rat heart

Immunogen:	A synthetic peptide of human UCP3.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	7352
Uniprot:	P55916
Cellular Location:	Mitochondrion inner membrane, Multi-pass membrane protein
Calculated MW:	22kDa/29kDa/34kDa
Observed MW:	34kDa

Synonyms:

UCP3, SLC25A9

Background:

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCPs have tissue-specific expression; this gene is primarily expressed in skeletal muscle. This gene's protein product is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of this gene increase when fatty acid supplies to mitochondria exceed their oxidation capacity and the protein enables the export of fatty acids from mitochondria. UCPs contain the three solcar protein domains typically found in MACPs. Two splice variants have been found for this gene.

UniProt Protein Function:	UCP3: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the form of heat. May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance. Defects in UCP3 may be involved in obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the mitochondrial carrier family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Mitochondrial; Membrane protein, multi-pass; Membrane protein, integral; Transporter Chromosomal Location of Human Ortholog: 11q13.4 Cellular Component: integral to membrane; mitochondrial inner membrane; mitochondrion Molecular Function:oxidative phosphorylation uncoupler activity; protein binding; structural constituent of ribosome; transporter activity Biological Process: aging; cellular metabolic process; cellular response to hormone stimulus; fatty acid metabolic process; lipid metabolic process; mitochondrial transport; proton transport; respiratory gaseous exchange; response to activity; response to cold; response to glucocorticoid stimulus; response to hypoxia; response to insulin stimulus; response to nutrient; response to superoxide; translation Disease: Obesity
NCBI Summary:	Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCPs have tissue-specific expression; this gene is primarily expressed in skeletal muscle. This gene's protein product is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of this gene increase when fatty acid supplies to mitochondria exceed their oxidation capacity and the protein enables the export of fatty acids from mitochondria. UCPs contain the three solcar protein domains typically found in MACPs. Two splice variants have been found for this gene.[provided by RefSeq, Nov 2008]
UniProt Code:	P55916
NCBI GenInfo Identifier:	2497983
NCBI Gene ID:	7352
NCBI Accession:	P55916.1
UniProt Secondary Accession:	P55916,O60475, Q96HL3,
UniProt Related Accession:	P55916
Molecular Weight:
NCBI Full Name:	Mitochondrial uncoupling protein 3
NCBI Synonym Full Names:	uncoupling protein 3 (mitochondrial, proton carrier)
NCBI Official Symbol:	UCP3
NCBI Official Synonym Symbols:	SLC25A9
NCBI Protein Information:	mitochondrial uncoupling protein 3
UniProt Protein Name:	Mitochondrial uncoupling protein 3
UniProt Synonym Protein Names:	Solute carrier family 25 member 9
Protein Family:	UBA domain-containing protein
UniProt Gene Name:	UCP3
UniProt Entry Name:	UCP3_HUMAN

	Western blot analysis of extracts of various cell lines, using STX17 antibody (CAB16995) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunohistochemistry of paraffin-embedded human liver using UCP3 Rabbit pAb (CAB16995) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse skeletal muscle using UCP3 Rabbit pAb (CAB16995) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-UCP3 Antibody (CAB1532)	Human Mitochondrial uncoupling protein 3 (UCP3) ELISA Kit
Anti-UCP3 Antibody (CAB16996)
UCP3 Antibody (PACO01677)
UCP3 Antibody (PACO13071)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)