Anti-UGT1A1 Antibody (CAB6186)

Antibody Name:	Anti-UGT1A1 Antibody
Antibody SKU:	CAB6186
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human UGT1A1 (NP_000454.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human UGT1A1 (NP_000454.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAVE SQGG RPLV LGLL LCVL GPVV SHAG KILL IPVD GSHW LSML GAIQ QLQQ RGHE IVVL APDA SLYI RDGA FYTL KTYP VPFQ REDV KESF VSLG HNVF ENDS FLQR VIKT YKKI KKDS AMLL SGCS HLLH NKEL MASL AESS FDVM LTDP FLPC SPIV AQYL SLPT VFFL HALP CSLE FEAT QCPN PFSY VPRP LSSH
Gene ID:	54658
Uniprot:	P22309
Cellular Location:	Endoplasmic reticulum, Endoplasmic reticulum membrane, Microsome, Single-pass membrane protein
Calculated MW:	49kDa/59kDa
Observed MW:	60kDa

Synonyms:

UGT1A1, BILIQTL1, GNT1, HUG-BR1, UDPGT, UDPGT 1-1, UGT1, UGT1A, UDPGT1-1

Background:

This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome.

UniProt Protein Function:	UGT1A1: UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform glucuronidates bilirubin IX- alpha to form both the IX-alpha-C8 and IX-alpha-C12 monoconjugates and diconjugate. Is also able to catalyze the glucuronidation of 17beta-estradiol, 17alpha-ethinylestradiol, 1-hydroxypyrene, 4- methylumbelliferone, 1-naphthol, paranitrophenol, scopoletin, and umbelliferone. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Expressed in liver. Not expressed in skin or kidney. Belongs to the UDP-glycosyltransferase family. 1 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Lipid Metabolism - androgen and estrogen; Xenobiotic Metabolism - drug metabolism - other enzymes; Xenobiotic Metabolism - metabolism by cytochrome P450; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Membrane protein, integral; Cofactor and Vitamin Metabolism - retinol; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - pentose and glucuronate interconversions; Carbohydrate Metabolism - ascorbate and aldarate; EC 2.4.1.17; Transferase Chromosomal Location of Human Ortholog: 2q37 Cellular Component: endoplasmic reticulum membrane; integral to plasma membrane; endoplasmic reticulum Molecular Function:enzyme inhibitor activity; protein homodimerization activity; retinoic acid binding; enzyme binding; glucuronosyltransferase activity; protein heterodimerization activity; steroid binding Biological Process: response to drug; steroid metabolic process; estrogen metabolic process; negative regulation of steroid metabolic process; organ regeneration; response to lipopolysaccharide; negative regulation of fatty acid metabolic process; liver development; cellular response to hormone stimulus; response to starvation; negative regulation of transferase activity; bilirubin conjugation; flavonoid biosynthetic process; heme catabolic process; xenobiotic metabolic process; negative regulation of catalytic activity; porphyrin metabolic process; digestion; flavone metabolic process; acute-phase response; retinoic acid metabolic process; heterocycle metabolic process; drug metabolic process; response to nutrient Disease: Gilbert Syndrome; Crigler-najjar Syndrome, Type I; Bilirubin, Serum Level Of, Quantitative Trait Locus 1; Crigler-najjar Syndrome, Type Ii; Hyperbilirubinemia, Transient Familial Neonatal
NCBI Summary:	This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome. [provided by RefSeq, Jul 2008]
UniProt Code:	P22309
NCBI GenInfo Identifier:	136729
NCBI Gene ID:	54658
NCBI Accession:	P22309.1
UniProt Secondary Accession:	P22309,A6NJC3, B8K286,
UniProt Related Accession:	P22309
Molecular Weight:	533
NCBI Full Name:	UDP-glucuronosyltransferase 1-1
NCBI Synonym Full Names:	UDP glucuronosyltransferase 1 family, polypeptide A1
NCBI Official Symbol:	UGT1A1
NCBI Official Synonym Symbols:	GNT1; UGT1; UDPGT; UGT1A; HUG-BR1; BILIQTL1; UDPGT 1-1
NCBI Protein Information:	UDP-glucuronosyltransferase 1-1; UGT-1A; UGT1*1; UGT1-01; UDP-glucuronosyltransferase 1-A; UDP-glucuronosyltransferase 1A1; bilirubin-specific UDPGT isozyme 1; bilirubin UDP-glucuronosyltransferase 1-1; bilirubin UDP-glucuronosyltransferase isozyme 1; UDP glycosyltransferase 1 family, polypeptide A1
UniProt Protein Name:	UDP-glucuronosyltransferase 1-1
UniProt Synonym Protein Names:	Bilirubin-specific UDPGT isozyme 1; hUG-BR1; UDP-glucuronosyltransferase 1-A; UGT-1A; UGT1A; UDP-glucuronosyltransferase 1A1
Protein Family:	UDP-glucuronosyltransferase
UniProt Gene Name:	UGT1A1
UniProt Entry Name:	UD11_HUMAN

	Western blot analysis of extracts of Rat liver, using UGT1A1 Rabbit pAb (CAB6186) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded Human liver cancer using UGT1A1 Rabbit pAb (CAB6186) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using UGT1A1 antibody (CAB6186). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of Rat liver using UGT1A1 antibody (CAB6186) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of Human liver cancer using UGT1A1 antibody (CAB6186) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-UGT1A1 Antibody (CAB1359)	Human UGT1A1 / UDP-glucuronosyltransferase 1-1 ELISA Kit
	Human UDP-glucuronosyltransferase 1-1 (UGT1A1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)