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Anti-ULk1 Antibody (CAB8529)

SKU:
CAB8529
Product Type:
Antibody
Applications:
WB
IHC
IF
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Autophagy
  • Autophagy Antibodies Anti-ULk1 Antibody CAB8529
  • Autophagy Antibodies Anti-ULk1 Antibody CAB8529
  • Autophagy Antibodies Anti-ULk1 Antibody CAB8529
  • Autophagy Antibodies Anti-ULk1 Antibody CAB8529
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-ULk1 Antibody
Antibody SKU:CAB8529
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 400-499 of human ULk1 (NP_003556.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 400-499 of human ULk1 (NP_003556.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RTPS PSPP CSSS PSPS GRAG PFSS SRCG ASVP IPVP TQVQ NYQR IERN LQSP TQFQ TPRS SAIR RSGS TSPL GFAR ASPS PPAH AEHG GVLA RKMS LGGG
Gene ID:8408
Uniprot:O75385
Cellular Location:Cytoplasm, Preautophagosomal structure, cytosol
Calculated MW:112kDa
Observed MW:150kDa
Synonyms:ULK1, ATG1, ATG1A, UNC51, Unc51.1, hATG1, ULk1
Background:
UniProt Protein Function:ULK1: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. Interacts with GABARAP and GABARAPL2. Interacts (via C- terminus) with ATG13/KIAA0652. Part of a complex consisting of ATG13/KIAA0652, ULK1 and RB1CC1. Associates with the mammalian target of rapamycin complex 1 (mTORC1) through an interaction with RPTOR; the association depends on nutrient conditions and is reduced during starvation. Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
UniProt Protein Details:

Protein type:Protein kinase, Other; Autophagy; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Other group; ULK family

Chromosomal Location of Human Ortholog: 12q24.3

Cellular Component: autophagic vacuole; cytosol; pre-autophagosomal structure membrane

Molecular Function:protein binding; protein complex binding; protein serine/threonine kinase activity; Rab GTPase binding

Biological Process: axon extension; cellular response to nutrient levels; macroautophagy; negative regulation of protein complex assembly; neurite development; peptidyl-serine phosphorylation; positive regulation of autophagy; positive regulation of macroautophagy; protein amino acid autophosphorylation; protein localization; regulation of autophagy; response to starvation

UniProt Code:O75385
NCBI GenInfo Identifier:317373288
NCBI Gene ID:8408
NCBI Accession:O75385.2
UniProt Secondary Accession:O75385,Q9UQ28,
UniProt Related Accession:O75385
Molecular Weight:112,631 Da
NCBI Full Name:Serine/threonine-protein kinase ULK1
NCBI Synonym Full Names:unc-51 like autophagy activating kinase 1
NCBI Official Symbol:ULK1  
NCBI Official Synonym Symbols:ATG1; ATG1A; UNC51; hATG1; Unc51.1  
NCBI Protein Information:serine/threonine-protein kinase ULK1
UniProt Protein Name:Serine/threonine-protein kinase ULK1
UniProt Synonym Protein Names:Autophagy-related protein 1 homolog; ATG1; hATG1; Unc-51-like kinase 1
Protein Family:Serine/threonine-protein kinase
UniProt Gene Name:ULK1  
UniProt Entry Name:ULK1_HUMAN
Anti-ULk1 Polyclonal Antibody (CAB8529)Western blot analysis of extracts of various cell lines, using ULK1 antibody (CAB8529) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
Anti-ULk1 Polyclonal Antibody (CAB8529)Immunohistochemistry of paraffin-embedded mouse liver using ULk1 antibody (CAB8529) at dilution of 1:100 (40x lens).
Anti-ULk1 Polyclonal Antibody (CAB8529)Immunohistochemistry of paraffin-embedded mouse heart using ULk1 antibody (CAB8529) at dilution of 1:100 (40x lens).
Anti-ULk1 Polyclonal Antibody (CAB8529)Immunofluorescence analysis of L929 cells using ULK1 antibody (CAB8529) at dilution of 1:100. Blue: DAPI for nuclear staining.

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