Anti-UPF2 Antibody (CAB7091)

Antibody Name:	Anti-UPF2 Antibody
Antibody SKU:	CAB7091
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1153-1272 of human UPF2 (NP_542166.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HT-29, Jurkat, HepG2, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1153-1272 of human UPF2 (NP_542166.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GPPL GGGE GEAE SADT MPFV MLTR KGNK QQFK ILNV PMSS QLAA NHWN QQQA EQEE RMRM KKLT LDIN ERQE QEDY QEML QSLA QRPA PANT NRER RPRY QHPK GAPN ADLI FKTG GRRR
Gene ID:	26019
Uniprot:	Q9HAU5
Cellular Location:	Cytoplasm, perinuclear region
Calculated MW:	66kDa/147kDa
Observed MW:	148kDa

Synonyms:

UPF2, HUPF2, RENT2, smg-3

Background:

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p. Two splice variants have been found for this gene; both variants encode the same protein.

UniProt Protein Function:	UPF2: Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC). Recruited by UPF3B associated with the EJC core at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF3B stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA. Found in a post-splicing messenger ribonucleoprotein (mRNP) complex. Associates with the exon junction complex (EJC). Interacts with SMG1, EST1A, UPF1, UPF3A, UPF3B, EIF4A1 and EIF1. Ubiquitous. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA-binding Chromosomal Location of Human Ortholog: 10p14-p13 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:protein binding; telomeric DNA binding Biological Process: mRNA catabolic process, nonsense-mediated decay; mRNA export from nucleus
NCBI Summary:	This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p. Two splice variants have been found for this gene; both variants encode the same protein. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9HAU5
NCBI GenInfo Identifier:	60390647
NCBI Gene ID:	26019
NCBI Accession:	Q9HAU5.1
UniProt Secondary Accession:	Q9HAU5,Q14BM1, Q5W0J4, Q8N8U1, Q9H1J2, Q9NWL1, Q9P2D9 Q9Y4M9, A6NLJ5, D3DRS0,
UniProt Related Accession:	Q9HAU5
Molecular Weight:	66,842 Da
NCBI Full Name:	Regulator of nonsense transcripts 2
NCBI Synonym Full Names:	UPF2 regulator of nonsense transcripts homolog (yeast)
NCBI Official Symbol:	UPF2
NCBI Official Synonym Symbols:	HUPF2; RENT2; smg-3
NCBI Protein Information:	regulator of nonsense transcripts 2
UniProt Protein Name:	Regulator of nonsense transcripts 2
UniProt Synonym Protein Names:	Nonsense mRNA reducing factor 2; Up-frameshift suppressor 2 homolog; hUpf2
Protein Family:	Regulator of nonsense transcripts
UniProt Gene Name:	UPF2
UniProt Entry Name:	RENT2_HUMAN

	Western blot analysis of extracts of various cell lines, using UPF2 antibody (CAB7091) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human lung using UPF2 antibody (CAB7091) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate using UPF2 antibody (CAB7091) at dilution of 1:100 (40x lens).

Antibodies

Anti-UPF2 Antibody (CAB13411)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)