Anti-USO1 Antibody (CAB16079)

Antibody Name:	Anti-USO1 Antibody
Antibody SKU:	CAB16079
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 663-962 of human USO1 (NP_003706.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Rat
Positive Samples:	U-87MG, Raji, SGC7901

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 663-962 of human USO1 (NP_003706.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DLQL EELR QQVS TLKC QNEQ LQTA VTQQ VSQI QQHK DQYN LLKI QLGK DNQH QGSY SEGA QMNG IQPE EIGR LREE IEEL KRNQ ELLQ SQLT EKDS MIEN MKSS QTSG TNEQ SSAI VSAR DSEQ VAEL KQEL ATLK SQLN SQSV EITK LQTE KQEL LQKT EAFA KSVE VQGE TETI IATK TTDV EGRL SALL QETK ELKN EIKA LSEE RTAI KEQL DSSN STIA ILQT EKDK LELE ITDS KKEQ DDLL VLLA DQDQ KILS LKNK LKDL GHPV EEED ELES GDQE DEDD ESED PGKD LDHI
Gene ID:	8615
Uniprot:	O60763
Cellular Location:	Cytoplasm, Golgi apparatus membrane, Peripheral membrane protein, cytosol
Calculated MW:	107kDa/109kDa
Observed MW:	108kDa

Synonyms:

USO1, P115, TAP, VDP

Background:

The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	TAP: General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity. Belongs to the VDP/USO1/EDE1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Vesicle Chromosomal Location of Human Ortholog: 4q21.1 Cellular Component: Golgi membrane; Golgi apparatus; Golgi stack; membrane; endoplasmic reticulum; perinuclear region of cytoplasm; nucleolus; cytosol Molecular Function:protein transporter activity Biological Process: intracellular protein transport; ER to Golgi vesicle-mediated transport; Golgi vesicle docking; vesicle fusion with Golgi apparatus; transcytosis; mitotic cell cycle
NCBI Summary:	The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
UniProt Code:	O60763
NCBI GenInfo Identifier:	143743108
NCBI Gene ID:	8615
NCBI Accession:	O60763.2
UniProt Related Accession:	O60763
Molecular Weight:
NCBI Full Name:	General vesicular transport factor p115
NCBI Synonym Full Names:	USO1 vesicle transport factor
NCBI Official Symbol:	USO1
NCBI Official Synonym Symbols:	TAP; VDP; P115
NCBI Protein Information:	general vesicular transport factor p115
UniProt Protein Name:	General vesicular transport factor p115
UniProt Synonym Protein Names:	Protein USO1 homolog; Transcytosis-associated protein; TAP; Vesicle-docking protein
Protein Family:	Tripeptidyl aminopeptidase
UniProt Gene Name:	USO1
UniProt Entry Name:	USO1_HUMAN

	Western blot analysis of extracts of various cell lines, using USO1 antibody (CAB16079) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of HeLa cells using USO1 antibody (CAB16079) at dilution of 1:200 (60x lens). Blue: DAPI for nuclear staining.
	Confocal immunofluorescence analysis of Hela cells using USO1 antibody (CAB16079) at dilution of 1:200. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-USO1 Antibody (CAB2796)	Human USO1 (Protein USO1 homolog) ELISA Kit (HUFI07377)
USO1 Antibody (PACO06717)
USO1 Antibody (PACO13113)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)