The USP5 Antibody (CAB4202) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit-derived antibody has high specificity for human samples and is validated for use in Western blotting applications. By binding to the USP5 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for investigations in the fields of molecular biology, biochemistry, and cancer research.USP5, also known as ubiquitin-specific protease 5, plays a crucial role in maintaining protein homeostasis by regulating the degradation of ubiquitinated proteins. Its functions in the ubiquitin-proteasome system have implications in various cellular processes, including DNA repair, cell cycle progression, and apoptosis.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
USP5 Antibody
SKU:
CAB4202
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).
Purification Method
Affinity purification
Gene ID
8078
RRID
AB_2765553
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using USP5 Rabbit pAb (CAB4202) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Rat stomach using USP5 Rabbit pAb (CAB4202) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using USP5 Rabbit pAb (CAB4202) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using USP5 Rabbit pAb (CAB4202) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of L929 cells using USP5 Rabbit pAb (CAB4202) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
