Anti-USP5 Antibody (CAB4202)

Antibody Name:	Anti-USP5 Antibody
Antibody SKU:	CAB4202
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human USP5 (NP_003472.2).

Application:	WB IHC
Recommended Dilution:	WB 1:1000 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, A-549, 293T, HeLa, Jurkat, 22Rv1, Mouse kidney, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human USP5 (NP_003472.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAEL SEEA LLSV LPTI RVPK AGDR VHKD ECAF SFDT PESE GGLY ICMN TFLG FGKQ YVER HFNK TGQR VYLH LRRT RRPK EEDP ATGT GDPP RKKP TRLA IGVE GGFD LSEE KFEL DEDV KIVI LPDY LEIA RDGL GGLP DIVR DRVT SAVE ALLS ADSA SRKQ EVQA WDGE VRQV SKHA FSLK QLDN PARI PPCG WKCS KCDM RENL WLNL TDGS ILCG RRYF DGSG GNNH AVEH YRET GYPL AVKL GTIT PDGA DVYS YDED DMVL DPSL AEHL SHFG
Gene ID:	8078
Uniprot:	P45974
Cellular Location:
Calculated MW:	93kDa/95kDa
Observed MW:	110kDa

Synonyms:

USP5, ISOT

Background:

Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM, Mar 2010]

UniProt Protein Function:	USP5: Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys- 63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition. Belongs to the peptidase C19 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.4.19.12; Ubiquitin-specific protease; Protease Chromosomal Location of Human Ortholog: 12p13 Cellular Component: lysosome Molecular Function:omega peptidase activity; protein binding; zinc ion binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of proteasomal ubiquitin-dependent protein catabolic process
NCBI Summary:	Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM, Mar 2010]
UniProt Code:	P45974
NCBI GenInfo Identifier:	1717869
NCBI Gene ID:	8078
NCBI Accession:	P45974.2
UniProt Related Accession:	P45974
Molecular Weight:	95786
NCBI Full Name:	Ubiquitin carboxyl-terminal hydrolase 5
NCBI Synonym Full Names:	ubiquitin specific peptidase 5
NCBI Official Symbol:	USP5
NCBI Official Synonym Symbols:	ISOT
NCBI Protein Information:	ubiquitin carboxyl-terminal hydrolase 5
UniProt Protein Name:	Ubiquitin carboxyl-terminal hydrolase 5
UniProt Synonym Protein Names:	Deubiquitinating enzyme 5; Isopeptidase T; Ubiquitin thioesterase 5; Ubiquitin-specific-processing protease 5
UniProt Gene Name:	USP5
UniProt Entry Name:	UBP5_HUMAN

	Western blot analysis of extracts of various cell lines, using USP5 antibody (CAB4202) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat stomach using USP5 Rabbit pAb (CAB4202) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using USP5 Rabbit pAb (CAB4202) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using USP5 Rabbit pAb (CAB4202) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using USP5 Rabbit pAb (CAB4202) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)