The VAPB Antibody (CAB5363) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, raised in rabbits, is highly reactive with human samples and is validated for use in various applications such as Western blot and immunofluorescence.VAPB is a key player in cellular processes such as lipid transport, ER stress response, and membrane trafficking. Dysregulation of VAPB has been linked to neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA).
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
VAPB Antibody
SKU:
CAB5363
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ALS8, VAP-B, VAMP-B, VAPB
Positive Sample:
Mouse lung, Mouse brain, Mouse kidney, Rat heart
Cellular Localization:
Endoplasmic Reticulum Membrane, Single-Pass Type Iv Membrane Protein.
Calculated MW:
27kDa
Observed MW:
27kDa
The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking.
Purification Method
Affinity purification
Gene ID
9217
RRID
AB_2766173
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using VAPB Rabbit pAb (CAB5363) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using VAPB Rabbit pAb (CAB5363) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of U2OS cells using VAPB Rabbit pAb (CAB5363) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
