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Anti-VCP Antibody (CAB13368)

SKU:
CAB13368
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Autophagy
  • Autophagy Antibodies Anti-VCP Antibody CAB13368
  • Autophagy Antibodies Anti-VCP Antibody CAB13368
  • Autophagy Antibodies Anti-VCP Antibody CAB13368
  • Autophagy Antibodies Anti-VCP Antibody CAB13368
  • Autophagy Antibodies Anti-VCP Antibody CAB13368
€139 - €419
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Description

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Antibody Name:Anti-VCP Antibody
Antibody SKU:CAB13368
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 647-806 of human VCP (NP_009057.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, K-562, SH-SY5Y, Mouse brain, Mouse spleen, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 647-806 of human VCP (NP_009057.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LPDE KSRV AILK ANLR KSPV AKDV DLEF LAKM TNGF SGAD LTEI CQRA CKLA IRES IESE IRRE RERQ TNPS AMEV EEDD PVPE IRRD HFEE AMRF ARRS VSDN DIRK YEMF AQTL QQSR GFGS FRFP SGNQ GGAG PSQG SGGG TGGS VYTE DNDD DLYG
Gene ID:7415
Uniprot:P55072
Cellular Location:Cytoplasm, Endoplasmic reticulum, Nucleus, cytosol
Calculated MW:89kDa
Observed MW:90KDa
Synonyms:VCP, ALS14, CDC48, CMT2Y, HEL-220, HEL-S-70, IBMPFD, IBMPFD1, TERA, p97
Background:The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.
UniProt Protein Function:VCP: valosin-containing protein (VCP) is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the nuclear envelope and of the transitional endoplasmic reticulum (tER). Regulates NFKappaB pathway, which is important for metastasis of osteosarcoma. Tyrosine phosphorylation regulates its cell cycle-dependent nuclear localization.
UniProt Protein Details:

Protein type:Endoplasmic reticulum; EC 3.6.4.6; DNA repair, damage; Chaperone; Hydrolase

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: nucleoplasm; proteasome complex; endoplasmic reticulum membrane; intracellular membrane-bound organelle; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; lipid particle; cytosol; nucleus

Molecular Function:protein domain specific binding; identical protein binding; protein binding; ATPase activity; protein complex binding; ADP binding; polyubiquitin binding; protein phosphatase binding; lipid binding; ATP binding; receptor binding

Biological Process: caspase activation; proteasomal ubiquitin-dependent protein catabolic process; ER to Golgi vesicle-mediated transport; ER-associated protein catabolic process; unfolded protein response; protein ubiquitination; DNA repair; regulation of apoptosis; retrograde protein transport, ER to cytosol; bypass DNA synthesis; establishment of protein localization; positive regulation of protein complex assembly; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; double-strand break repair; positive regulation of protein catabolic process; protein amino acid N-linked glycosylation via asparagine; response to DNA damage stimulus; protein homooligomerization

Disease: Amyotrophic Lateral Sclerosis 14, With Or Without Frontotemporal Dementia; Inclusion Body Myopathy With Early-onset Paget Disease With Or Without Frontotemporal Dementia 1

NCBI Summary:The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq, Jul 2008]
UniProt Code:P55072
NCBI GenInfo Identifier:6094447
NCBI Gene ID:7415
NCBI Accession:P55072.4
UniProt Secondary Accession:P55072,Q0V924, Q2TAI5, Q969G7, Q9UCD5, B2R5T8,
UniProt Related Accession:P55072
Molecular Weight:89,322 Da
NCBI Full Name:Transitional endoplasmic reticulum ATPase
NCBI Synonym Full Names:valosin containing protein
NCBI Official Symbol:VCP  
NCBI Official Synonym Symbols:p97; TERA; ALS14; IBMPFD  
NCBI Protein Information:transitional endoplasmic reticulum ATPase; TER ATPase; yeast Cdc48p homolog; valosin-containing protein; 15S Mg(2+)-ATPase p97 subunit
UniProt Protein Name:Transitional endoplasmic reticulum ATPase
UniProt Synonym Protein Names:15S Mg(2+)-ATPase p97 subunit; Valosin-containing protein
Protein Family:Transitional endoplasmic reticulum ATPase
UniProt Gene Name:VCP  
UniProt Entry Name:TERA_HUMAN
Anti-VCP Antibody (CAB13368)Western blot analysis of extracts of various cell lines, using VCP antibody (CAB13368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-VCP Antibody (CAB13368)Immunohistochemistry of paraffin-embedded human placenta using VCP Rabbit pAb (CAB13368) at dilution of 1:100 (40x lens).
Anti-VCP Antibody (CAB13368)Immunohistochemistry of paraffin-embedded mouse spinal cord using VCP Rabbit pAb (CAB13368) at dilution of 1:100 (40x lens).
Anti-VCP Antibody (CAB13368)Immunohistochemistry of paraffin-embedded rat kidney using VCP Rabbit pAb (CAB13368) at dilution of 1:100 (40x lens).
Anti-VCP Antibody (CAB13368)Immunofluorescence analysis of C6 cells using VCP antibody (CAB13368) at dilution of 1:100. Blue: DAPI for nuclear staining.

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