Description
VEEV Spike glycoprotein E2 Recombinant Protein (N-His)
VEEV Spike glycoprotein E2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ser335-Pro679, with an N-terminal His tag. Predicted molecular weight: 40.72 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Structural polyprotein is a ~138 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. For research use only.
The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.
| Product Name: | VEEV Spike glycoprotein E2 Recombinant Protein (N-His) |
| SKU: | GXRP10387 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | VEEV |
| Synonyms: | E2 envelope glycoprotein, Spike glycoprotein E2, pE2 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ser335-Pro679 |
| Calculated MW: | 40.72 kDa |
| UniProt: | P09592 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
