The VPS18 Polyclonal Antibody (CAB16654) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is highly reactive with human samples and is validated for use in Western blot applications. It binds to the VPS18 protein, allowing for the detection and analysis of VPS18 in various cell types, making it ideal for studies in cell biology and vesicle trafficking research.VPS18 is a crucial component of the ESCRT pathway, which is involved in the sorting and trafficking of proteins within the cell.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
VPS18 Polyclonal Antibody
SKU:
CAB16654
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:1000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PEP3, VPS18
Positive Sample:
A-549, mouse brain, rat brain
Cellular Localization:
Autophagosome, Clathrin-Coated Vesicle, Corvet Complex, Early Endosome, Endosome, Glutamatergic Synapse, Late Endosome, Lysosome, Presynapse.
Calculated MW:
110kDa
Observed MW:
110kDa
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps18 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.
Purification Method
Affinity purification
Gene ID
57617
RRID
AB_2772867
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using VPS18 Rabbit pAb (CAB16654) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 4min.
Immunofluorescence analysis of C6 cells using VPS18 Rabbit pAb (CAB16654) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using VPS18 Rabbit pAb (CAB16654) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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