WDR45 Antibody is a rabbit polyclonal antibody directed against WDR45, offered by Assay Genie for research applications. Reported applications include WB and ICC/IF. Reported reactivity: Human and Rat. Purified by Peptide Affinity.
WDR45, also known as WIPI-4, is a 360-amino acid protein containing WD40 repeats, which are structural motifs involved in protein-protein interactions. As a member of the WIPI (WD repeat domain phosphoinositide-interacting) protein family, WDR45 plays a critical role in autophagy—a cellular degradation and recycling process essential for neuronal health and homeostasis. WDR45 exists in three alternatively spliced isoforms and is ubiquitously expressed, with highest levels in heart and skeletal muscle. In the nervous system, WDR45 is vital for maintaining cellular quality control, particularly in long-lived neurons that are highly susceptible to proteotoxic stress. Dysfunction in WDR45-mediated autophagy has been directly linked to neurodegeneration with brain iron accumulation (NBIA), specifically types 4 and 5, which are characterized by progressive cognitive decline, motor dysfunction, and iron deposition in the basal ganglia. Mutations in the WDR45 gene disrupt autophagosome formation and trafficking, leading to the accumulation of damaged proteins and organelles—a hallmark of many neurodegenerative diseases, including Parkinson’s and Alzheimer’s. As such, WDR45 is increasingly recognized as a potential biomarker and therapeutic target in neurodegenerative disease research. Understanding the molecular mechanisms of WDR45 offers valuable insights into the intersection of autophagy, iron metabolism, and neuronal survival. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 100 µg. For research use only; not for diagnostic or therapeutic procedures.
Product Name:
WDR45 Antibody
Product SKU:
STPA00065
Size:
100 µg
Target:
WDR45
Host Species:
Rabbit
Antibody Type:
Polyclonal
Research Areas:
Cancer, Autophagy, Cell Signaling, Apoptosis
Buffer:
PBS, 50% glycerol, 0.09% sodium azide
Storage & Shipping:
Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:
Liquid
Applications:
WB, ICC/IF
Purification:
Peptide Affinity Purified
Concentration:
1 mg/ml
Reactivity:
Human, Rat
Recommended Dilution:
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Specificity:
Detects ~40 kDa.
Guarantee:
12 months from date of dispatch
Immunogen:
Synthetic peptide from the C-terminal of Human WIPI4 (aa. 312-322)
Immunogen Species:
Human
Accession Number:
NP_001025067.1
Gene ID:
11152
Swiss-Prot:
Q9Y484
Tissue Specificity:
Ubiquitously expressed, with high expression in skeletal muscle and heart. Weakly expressed in liver and placenta. Expression is down-regulated in pancreatic and in kidney tumors.
Alternative Names:
WDR45, WD Repeat Domain 45, WIPI4, WIPI-4, WD Repeat Domain Phosphoinositide-Interacting Protein 4, WD Repeat-Containing Protein 45, WD Repeat Domain X-Linked 1, WDRX1, WDRXI4, JM5, NBIA4, NBIA5, WD45 Repeat Protein Interacting With Phosphoinositides, Progranulin-Associated Neurodegeneration Protein, MGC163339, MGC163341
References:
1. http://www.genecards.org/cgi-bin/carddisp.pl?gene=WDR45 2. Strausberg R.L., et al. (2002) Proc Natl Acad Sci U S A. 99(26): 16899–16903. 3. Haack T.B., et al. (2012) Am J Hum Genet. 91(6): 1144–1149.
