Anti-WIF1 Antibody (CAB12969)

Antibody Name:	Anti-WIF1 Antibody
Antibody SKU:	CAB12969
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 29-220 of human WIF1 (NP_009122.2).

Application:	WB IHC
Recommended Dilution:	WB 1:1000 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 29-220 of human WIF1 (NP_009122.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GPPQ EESL YLWI DAHQ ARVL IGFE EDIL IVSE GKMA PFTH DFRK AQQR MPAI PVNI HSMN FTWQ AAGQ AEYF YEFL SLRS LDKG IMAD PTVN VPLL GTVP HKAS VVQV GFPC LGKQ DGVA AFEV DVIV MNSE GNTI LQTP QNAI FFKT CQQA ECPG GCRN GGFC NERR ICEC PDGF HGPH CEKA LCTP RCMN
Gene ID:	11197
Uniprot:	Q9Y5W5
Cellular Location:	Secreted
Calculated MW:	41kDa
Observed MW:	42kDa

Synonyms:

WIF1, WIF-1

Background:

The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.

UniProt Protein Function:	WIF1: Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.
UniProt Protein Details:	Protein type:Inhibitor; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 12q14.3 Molecular Function:protein binding
NCBI Summary:	The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers. [provided by RefSeq, Jun 2010]
UniProt Code:	Q9Y5W5
NCBI GenInfo Identifier:	61252765
NCBI Gene ID:	11197
NCBI Accession:	Q9Y5W5.3
UniProt Secondary Accession:	Q9Y5W5,Q6UXI1, Q8WVG4,
UniProt Related Accession:	Q9Y5W5
Molecular Weight:	41,528 Da
NCBI Full Name:	Wnt inhibitory factor 1
NCBI Synonym Full Names:	WNT inhibitory factor 1
NCBI Official Symbol:	WIF1
NCBI Official Synonym Symbols:	WIF-1
NCBI Protein Information:	wnt inhibitory factor 1
UniProt Protein Name:	Wnt inhibitory factor 1
Protein Family:	Wnt inhibitory factor
UniProt Gene Name:	WIF1
UniProt Entry Name:	WIF1_HUMAN

	Western blot analysis of extracts of mouse heart, using WIF1 antibody (CAB12969) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded Human breast cancer using WIF1 Rabbit pAb (CAB12969) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-WIF1 Antibody (CAB5386)	Human WIF1 ELISA Kit
Anti-WIF1 Antibody (CAB5171)	Human Wnt inhibitory factor 1 (WIF1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)