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Anti-TAZ Antibody (CAB15806)

SKU:
CAB15806
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 2 Anti-TAZ Antibody CAB15806
  • Epigenetics and Nuclear Signaling Antibodies 2 Anti-TAZ Antibody CAB15806
€139 - €419
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Description

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Antibody Name:Anti-TAZ Antibody
Antibody SKU:CAB15806
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human TAZ (NP_056287.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:NCI-H460, Mouse lung
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human TAZ (NP_056287.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LNGG PYHS REQS TDSG LGLG CYSV PTTP EDFL SNVD EMDT GENA GQTP MNIN PQQT RFPD FLDC LPGT NVDL GTLE SEDL IPLF NDVE SALN KSEP FLTW L
Gene ID:25937
Uniprot:Q9GZV5
Cellular Location:Cytoplasm, Nucleus
Calculated MW:44kDa
Observed MW:55kDa
Synonyms:WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif, TAZ, WWTR1
Background:
UniProt Protein Function:TAZ: Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition. Binds to SLC9A3R2 via the PDZ motif at the plasma membrane. Binds to YWHAZ in vivo and in vitro through the phosphoserine-binding motif RSHSSP. Interacts (via coiled-coil domain) with SMAD2 (via MH1 domain), SMAD3 and SMAD4. Interacts with MED15, PAX8 and NKX2-1. Interacts with TEAD1, TEAD2, TEAD3 and TEAD4. Highly expressed in kidney, heart, placenta and lung. Expressed in the thyroid tissue.
UniProt Protein Details:

Protein type:Oncoprotein; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 3q23-q24

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function:protein binding; transcription coactivator activity

Biological Process: negative regulation of protein amino acid phosphorylation; negative regulation of protein kinase activity; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; stem cell division; transcription initiation from RNA polymerase II promoter

UniProt Code:Q9GZV5
NCBI GenInfo Identifier:67462080
NCBI Gene ID:25937
NCBI Accession:Q9GZV5.1
UniProt Secondary Accession:Q9GZV5,Q8N3P2, Q9Y3W6, D3DNH7,
UniProt Related Accession:Q9GZV5
Molecular Weight:44,101 Da
NCBI Full Name:WW domain-containing transcription regulator protein 1
NCBI Synonym Full Names:WW domain containing transcription regulator 1
NCBI Official Symbol:WWTR1  
NCBI Official Synonym Symbols:TAZ  
NCBI Protein Information:WW domain-containing transcription regulator protein 1
UniProt Protein Name:WW domain-containing transcription regulator protein 1
UniProt Synonym Protein Names:Transcriptional coactivator with PDZ-binding motif
Protein Family:WW domain-containing transcription regulator protein
UniProt Gene Name:WWTR1  
UniProt Entry Name:WWTR1_HUMAN
Anti-TAZ Antibody (CAB15806)Western blot analysis of extracts of various cell lines, using TAZ antibody (CAB15806) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-TAZ Antibody (CAB15806)Immunofluorescence analysis of U2OS cells using TAZ antibody (CAB15806) at dilution of 1:100. Blue: DAPI for nuclear staining.

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