Western blot analysis of various lysates, using TAZ antibody (CAB23034) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The TAZ Monoclonal Antibody (CAB23034) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through a monoclonal antibody production process, demonstrates high specificity and sensitivity in detecting TAZ protein levels in human samples. Validated for use in various applications, including Western blot and immunohistochemistry, it enables precise analysis of TAZ expression in different cell types.TAZ, also known as WW domain-containing transcription regulator 1, is involved in diverse cellular processes such as cell proliferation, differentiation, and stem cell self-renewal. Dysregulation of TAZ has been linked to numerous diseases, including cancer, cardiovascular disorders, and developmental abnormalities.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TAZ Monoclonal Antibody
SKU:
CAB23034
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC57803
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TAZ
Positive Sample:
HeLa, K-562(Negative control), NIH/3T3, Mouse lung, Rat lung
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
44kDa
Observed MW:
55kDa
Enables transcription coactivator activity. Involved in several processes, including hippo signaling; positive regulation of cell differentiation; and regulation of signal transduction. Located in cytosol and nuclear body.
Purification Method
Affinity purification
Gene ID
25937
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using TAZ Rabbit mAb (CAB23034) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of various lysates, using TAZ Rabbit mAb (CAB23034) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
