Anti-XRCC2 Antibody (CAB1800)

Antibody Name:	Anti-XRCC2 Antibody
Antibody SKU:	CAB1800
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human XRCC2 (NP_005422.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	HeLa, K-562, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human XRCC2 (NP_005422.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MCSA FHRA ESGT ELLA RLEG RSSL KEIE PNLF ADED SPVH GDIL EFHG PEGT GKTE MLYH LTAR CILP KSEG GLEV EVLF IDTD YHFD MLRL VTIL EHRL SQSS EEII KYCL GRFF LVYC SSST HLLL TLYS LESM FCSH PSLC LLIL DSLS AFYW IDRV NGGE SVNL QEST LRKC SQCL EKLV NDYR LVLF ATTQ TIMQ KASS SSEE PSHA SRRL CDVD IDYR PYLC KAWQ QLVK HRMF FSKQ DDSQ SSNQ FSLV SRCL KSNS LKKH FFII GESG VEFC
Gene ID:	7516
Uniprot:	O43543
Cellular Location:	Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:	31kDa
Observed MW:	37kDa

Synonyms:

XRCC2, FANCU

Background:

This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene is involved in the repair of DNA double-strand breaks by homologous recombination and it functionally complements Chinese hamster irs1, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents.

UniProt Protein Function:	XRCC2: Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Belongs to the RecA family. RAD51 subfamily.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 7q36.1 Cellular Component: centrosome; nucleoplasm; replication fork Molecular Function:DNA-dependent ATPase activity; double-stranded DNA binding; endodeoxyribonuclease activity; four-way junction DNA binding; protein binding; recombinase activity; single-stranded DNA binding Biological Process: centrosome organization and biogenesis; DNA repair; DNA synthesis during DNA repair; double-strand break repair via homologous recombination; meiosis; meiotic DNA recombinase assembly; meiotic recombination; mitotic cell cycle; mitotic recombination; response to ionizing radiation; strand displacement; strand invasion
NCBI Summary:	This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene is involved in the repair of DNA double-strand breaks by homologous recombination and it functionally complements Chinese hamster irs1, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents. [provided by RefSeq, Jul 2008]
UniProt Code:	O43543
NCBI GenInfo Identifier:	20140429
NCBI Gene ID:	7516
NCBI Accession:	O43543.1
UniProt Secondary Accession:	O43543,B2R925,
UniProt Related Accession:	O43543
Molecular Weight:	31,956 Da
NCBI Full Name:	DNA repair protein XRCC2
NCBI Synonym Full Names:	X-ray repair cross complementing 2
NCBI Official Symbol:	XRCC2
NCBI Official Synonym Symbols:	FANCU
NCBI Protein Information:	DNA repair protein XRCC2
UniProt Protein Name:	DNA repair protein XRCC2
UniProt Synonym Protein Names:	X-ray repair cross-complementing protein 2
Protein Family:	DNA repair protein
UniProt Gene Name:	XRCC2
UniProt Entry Name:	XRCC2_HUMAN

	Western blot analysis of extracts of various cell lines, using XRCC2 antibody (CAB1800) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human thyroid cancer using XRCC2 antibody (CAB1800) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of MCF-7 cells using XRCC2 antibody (CAB1800). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of A549 cells using XRCC2 antibody (CAB1800).

Antibodies

Anti-XRCC2 Antibody (CAB13639)

XRCC2 Antibody (PACO01709)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)