Anti-XRCC3 Antibody (CAB2134)

Antibody Name:	Anti-XRCC3 Antibody
Antibody SKU:	CAB2134
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 62-346 of human XRCC3 (NP_001093588.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 62-346 of human XRCC3 (NP_001093588.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ILTA LQLH QQKE RFPT QHQR LSLG CPVL DALL RGGL PLDG ITEL AGRS SAGK TQLA LQLC LAVQ FPRQ HGGL EAGA VYIC TEDA FPHK RLQQ LMAQ QPRL RTDV PGEL LQKL RFGS QIFI EHVA DVDT LLEC VNKK VPVL LSRG MARL VVID SVAA PFRC EFDS QASA PRAR HLQS LGAT LREL SSAF QSPV LCIN QVTE AMEE QGAA HGPL GFWD ERVS PALG ITWA NQLL VRLL ADRL REEE AALG CPAR TLRV LSAP HLPP SSCS YTIS AEGV RGTP GTQS H
Gene ID:	7517
Uniprot:	O43542
Cellular Location:	Cytoplasm, Mitochondrion, Nucleus, perinuclear region
Calculated MW:	37kDa
Observed MW:	37kDa

Synonyms:

XRCC3, CMM6

Background:

This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Alternatively spliced transcript variants encoding the same protein have been identified.

UniProt Protein Function:	XRCC3: Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C. Defects in XRCC3 are the cause of susceptibility to breast cancer (BC). BC is a common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case. Defects in XRCC3 are the cause of susceptibility to cutaneous malignant melanoma type 6 (CMM6). CMM6 is a malignant neoplasm of melanocytes, arising de novo or from a pre- existing benign nevus, which occurs most often in the skin but also may involve other sites. Belongs to the RecA family. RAD51 subfamily.
UniProt Protein Details:	Protein type:DNA repair, damage Chromosomal Location of Human Ortholog: 14q32.3 Cellular Component: mitochondrion; perinuclear region of cytoplasm; cytoplasm; replication fork; nucleus Molecular Function:DNA-dependent ATPase activity; protein binding; four-way junction DNA binding; crossover junction endodeoxyribonuclease activity; ATP binding Biological Process: response to organic substance; DNA repair; DNA catabolic process, endonucleolytic; response to DNA damage stimulus; double-strand break repair via homologous recombination; DNA recombination Disease: Melanoma, Cutaneous Malignant, Susceptibility To, 6; Breast Cancer
NCBI Summary:	This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	O43542
NCBI GenInfo Identifier:	20140428
NCBI Gene ID:	7517
NCBI Accession:	O43542.1
UniProt Secondary Accession:	O43542,O43568, Q9BU18,
UniProt Related Accession:	O43542
Molecular Weight:	37,850 Da
NCBI Full Name:	DNA repair protein XRCC3
NCBI Synonym Full Names:	X-ray repair complementing defective repair in Chinese hamster cells 3
NCBI Official Symbol:	XRCC3
NCBI Official Synonym Symbols:	CMM6
NCBI Protein Information:	DNA repair protein XRCC3; X-ray repair cross-complementing protein 3
UniProt Protein Name:	DNA repair protein XRCC3
UniProt Synonym Protein Names:	X-ray repair cross-complementing protein 3
Protein Family:	DNA repair protein
UniProt Gene Name:	XRCC3
UniProt Entry Name:	XRCC3_HUMAN

	Western blot analysis of extracts of HL-60 cells, using XRCC3 antibody (CAB2134) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat testis using XRCC3 antibody (CAB2134) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using XRCC3 antibody (CAB2134) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using XRCC3 antibody (CAB2134) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using XRCC3 antibody (CAB2134). Blue: DAPI for nuclear staining.

Antibodies

XRCC3 Antibody (PACO01710)

XRCC3 Antibody (PACO05312)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)