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Immunology Antibodies 1 Anti-YTHDF1 Antibody CAB13260
Key Features

Anti-YTHDF1 Antibody (CAB13260)

Datasheet MSDS
SKU CAB13260
Product Type Antibody
Antibody Type Polyclonal Antibody
Reactivity Human, Mouse, Rat
Host Species Rabbit
Synonyms DF1, C20orf21, YTHDF1
Research Use Immunology
€96 - €370

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Description

system_update_altDatasheetsystem_update_altMSDS

YTHDF1 Polyclonal Antibody (CAB13260)

The YTHDF1 Polyclonal Antibody (CAB13260) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting YTHDF1 in human samples, making it suitable for various applications such as Western blot and immunohistochemistry.YTHDF1 is a member of the YTH domain-containing family of proteins, which play important roles in mRNA decay and translation regulation. As a reader protein for N6-methyladenosine (m6A) modifications on RNA molecules, YTHDF1 is involved in various biological processes, including cell differentiation, development, and cancer progression.

This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:YTHDF1 Polyclonal Antibody
SKU:CAB13260
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MSAT SVDT QRTK GQDN KVQN GSLH QKDT VHDN DFEP YLTG QSNQ SNSY PSMS DPYL SSYY PPSI GFPY SLNE APWS TAGD PPIP YLTT YGQL SNGD HHFM HDAV FGQP GGLG NNIY QHRF NFFP ENPA FSAW GTSG SQGQ QTQS SAYG SSYT YPPS SLGG TVVD GQPG FHSD TLSK APGM NSLE QGMV GLKI GDVS SSAV
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:
WB1:100 - 1:500
IF/ICC1:50 - 1:200
IP1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:DF1, C20orf21, YTHDF1
Positive Sample:Jurkat, C6
Cellular Localization:Cytoplasm.
Calculated MW:61kDa
Observed MW:70kDa

Enables N6-methyladenosine-containing RNA binding activity and ribosome binding activity. Involved in mRNA destabilization; positive regulation of translational initiation; and stress granule assembly. Located in P-body and cytoplasmic stress granule.

Purification MethodAffinity purification
Gene ID54915
RRIDAB_2760113
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using YTHDF1 Rabbit pAb (CAB13260) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageImmunofluorescence analysis of L929 cells using YTHDF1 Rabbit pAb (CAB13260) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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