The ZCCHC3 Antibody (CAB17235) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and exhibits high reactivity with human samples, making it ideal for Western blot applications. By binding to the ZCCHC3 protein, this antibody enables the detection and analysis of ZCCHC3 in various cell types, providing valuable insights into RNA processing and gene expression regulation.ZCCHC3 is known to play a role in post-transcriptional modifications of RNA, including mRNA decay and processing. Its functions are crucial for maintaining proper gene expression and cellular functions, making it a key target for research in fields such as molecular biology, genetics, and biotechnology.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ZCCHC3 Antibody
SKU:
CAB17235
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
C20orf99, ZCCHC3
Positive Sample:
LO2, mouse liver
Cellular Localization:
Cytoplasm.
Calculated MW:
44kDa
Observed MW:
43kDa
Enables double-stranded DNA binding activity. Involved in several processes, including cellular response to exogenous dsRNA; detection of virus; and positive regulation of RIG-I signaling pathway. Located in cytoplasm.
Purification Method
Affinity purification
Gene ID
85364
RRID
AB_2772945
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ZCCHC3 Rabbit pAb (CAB17235) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
Immunofluorescence analysis of L929 cells using ZCCHC3 Rabbit pAb (CAB17235) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
