Anti-ZEB1 Antibody (CAB16981)
- SKU:
- CAB16981
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
Anti-ZEB1 Antibody (CAB16981)
The ZEB1 Polyclonal Antibody (CAB16981) is a valuable tool for researchers studying the ZEB1 protein, a transcription factor that plays a crucial role in regulating cellular processes such as proliferation, differentiation, and migration. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.ZEB1 is known for its involvement in epithelial-to-mesenchymal transition (EMT), a process where cells lose their epithelial characteristics and gain mesenchymal properties, allowing for enhanced cell motility and invasiveness.
Dysregulation of ZEB1 has been linked to various diseases, including cancer, fibrosis, and developmental disorders.By targeting the ZEB1 protein, researchers can gain insights into the mechanisms underlying EMT and its role in disease progression. The ZEB1 Polyclonal Antibody is an essential tool for conducting studies in cancer biology, tissue remodeling, and developmental biology, providing valuable information for potential therapeutic interventions targeting ZEB1-mediated pathways.
| Antibody Name: | Anti-ZEB1 Antibody |
| Antibody SKU: | CAB16981 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human ZEB1. |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Jurkat |
| Immunogen: | A synthetic peptide of human ZEB1. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 6935 |
| Uniprot: | P37275 |
| Cellular Location: | |
| Calculated MW: | 116kDa/122kDa/124kDa |
| Observed MW: | 180kDa |
| Synonyms: | ZEB1, AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP, ZFHX1A |
| Background: | This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described. |
| UniProt Protein Function: | TCF8: Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. Interacts (via N-terminus) with SMARCA4/BRG1. Colocalizes with SMARCA4/BRG1 in E-cadherin- negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas. Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family. |
| UniProt Protein Details: | Protein type:C2H2-type zinc finger protein; DNA-binding; Transcription, coactivator/corepressor; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 10p11.2 Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus Molecular Function:protein binding; zinc ion binding; double-stranded DNA binding; transcription coactivator activity; chromatin binding; transcription factor binding; transcription factor activity; transcription corepressor activity Biological Process: response to nutrient levels; transcription, DNA-dependent; regulation of T cell differentiation in the thymus; semicircular canal morphogenesis; negative regulation of epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; pattern specification process; embryonic skeletal morphogenesis; regulation of transcription from RNA polymerase II promoter; regulation of mesenchymal cell proliferation; cell proliferation; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; cartilage development; regulation of smooth muscle cell differentiation; forebrain development; positive regulation of transcription from RNA polymerase II promoter; immune response; response to activity; embryonic camera-type eye morphogenesis; negative regulation of transcription, DNA-dependent; positive regulation of neuron differentiation Disease: Corneal Dystrophy, Posterior Polymorphous, 3; Corneal Dystrophy, Fuchs Endothelial, 6 |
| NCBI Summary: | This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010] |
| UniProt Code: | P37275 |
| NCBI GenInfo Identifier: | 6166575 |
| NCBI Gene ID: | 6935 |
| NCBI Accession: | P37275.2 |
| UniProt Secondary Accession: | P37275,Q12924, Q13800, Q2KJ05, Q5T968, Q5VZ84, Q8NB68 B4DJV0, B4DUW9, E9PCM7, F5H4I8, |
| UniProt Related Accession: | P37275 |
| Molecular Weight: | 122,084 Da |
| NCBI Full Name: | Zinc finger E-box-binding homeobox 1 |
| NCBI Synonym Full Names: | zinc finger E-box binding homeobox 1 |
| NCBI Official Symbol: | ZEB1Â Â |
| NCBI Official Synonym Symbols: | BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1Â Â |
| NCBI Protein Information: | zinc finger E-box-binding homeobox 1; negative regulator of IL2; delta-crystallin enhancer binding factor 1; posterior polymorphous corneal dystrophy 3; zinc finger homeodomain enhancer-binding protein; transcription factor 8 (represses interleukin 2 expression) |
| UniProt Protein Name: | Zinc finger E-box-binding homeobox 1 |
| UniProt Synonym Protein Names: | NIL-2-A zinc finger protein; Negative regulator of IL2; Transcription factor 8; TCF-8 |
| Protein Family: | Zinc finger E-box-binding homeobox |
| UniProt Gene Name: | ZEB1Â Â |
| UniProt Entry Name: | ZEB1_HUMAN |
 | Western blot analysis of extracts of various cells, using ZEB1 antibody (CAB16981) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5min. |
 | Immunohistochemistry of paraffin-embedded rat ovary using ZEB1 antibody (CAB16981) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast cancer using ZEB1 antibody (CAB16981) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse liver using ZEB1 antibody (CAB16981) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using ZEB1 antibody (CAB16981) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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