Anti-ZEB1 Antibody (CAB16981)

Antibody Name:	Anti-ZEB1 Antibody
Antibody SKU:	CAB16981
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human ZEB1.

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat

Immunogen:	A synthetic peptide of human ZEB1.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	6935
Uniprot:	P37275
Cellular Location:
Calculated MW:	116kDa/122kDa/124kDa
Observed MW:	180kDa

Synonyms:	ZEB1, AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP, ZFHX1A
Background:	This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.

UniProt Protein Function:	TCF8: Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. Interacts (via N-terminus) with SMARCA4/BRG1. Colocalizes with SMARCA4/BRG1 in E-cadherin- negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas. Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
UniProt Protein Details:	Protein type:C2H2-type zinc finger protein; DNA-binding; Transcription, coactivator/corepressor; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 10p11.2 Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus Molecular Function:protein binding; zinc ion binding; double-stranded DNA binding; transcription coactivator activity; chromatin binding; transcription factor binding; transcription factor activity; transcription corepressor activity Biological Process: response to nutrient levels; transcription, DNA-dependent; regulation of T cell differentiation in the thymus; semicircular canal morphogenesis; negative regulation of epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; pattern specification process; embryonic skeletal morphogenesis; regulation of transcription from RNA polymerase II promoter; regulation of mesenchymal cell proliferation; cell proliferation; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; cartilage development; regulation of smooth muscle cell differentiation; forebrain development; positive regulation of transcription from RNA polymerase II promoter; immune response; response to activity; embryonic camera-type eye morphogenesis; negative regulation of transcription, DNA-dependent; positive regulation of neuron differentiation Disease: Corneal Dystrophy, Posterior Polymorphous, 3; Corneal Dystrophy, Fuchs Endothelial, 6
NCBI Summary:	This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010]
UniProt Code:	P37275
NCBI GenInfo Identifier:	6166575
NCBI Gene ID:	6935
NCBI Accession:	P37275.2
UniProt Secondary Accession:	P37275,Q12924, Q13800, Q2KJ05, Q5T968, Q5VZ84, Q8NB68 B4DJV0, B4DUW9, E9PCM7, F5H4I8,
UniProt Related Accession:	P37275
Molecular Weight:	122,084 Da
NCBI Full Name:	Zinc finger E-box-binding homeobox 1
NCBI Synonym Full Names:	zinc finger E-box binding homeobox 1
NCBI Official Symbol:	ZEB1
NCBI Official Synonym Symbols:	BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1
NCBI Protein Information:	zinc finger E-box-binding homeobox 1; negative regulator of IL2; delta-crystallin enhancer binding factor 1; posterior polymorphous corneal dystrophy 3; zinc finger homeodomain enhancer-binding protein; transcription factor 8 (represses interleukin 2 expression)
UniProt Protein Name:	Zinc finger E-box-binding homeobox 1
UniProt Synonym Protein Names:	NIL-2-A zinc finger protein; Negative regulator of IL2; Transcription factor 8; TCF-8
Protein Family:	Zinc finger E-box-binding homeobox
UniProt Gene Name:	ZEB1
UniProt Entry Name:	ZEB1_HUMAN

	Western blot analysis of extracts of various cells, using ZEB1 antibody (CAB16981) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5min.
	Immunohistochemistry of paraffin-embedded rat ovary using ZEB1 antibody (CAB16981) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using ZEB1 antibody (CAB16981) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using ZEB1 antibody (CAB16981) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using ZEB1 antibody (CAB16981) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-ZEB1 Antibody (CAB1500)[KO Validated]	Human ZEB1 / Zinc finger E-box-binding homeobox 2 ELISA Kit
Anti-ZEB1 Antibody (CAB5600)	Human Zinc finger E-box-binding homeobox 1 (ZEB1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)