Western blot analysis of extracts from normal (control) and ZMYND8 knockout (KO) HeLa cells, using ZMYND8 antibody (CAB21848) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The ZMYND8 Polyclonal Antibody (CAB21848) is a high-quality antibody developed for reliable detection and analysis of target proteins. ZMYND8 is a key player in epigenetic regulation, functioning as a reader of methylated histones and playing a role in transcriptional regulation.This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in various applications such as Western blot and immunohistochemistry. Its ability to bind specifically to the ZMYND8 protein allows for accurate detection and analysis in a wide range of cell types, making it an essential tool for researchers studying epigenetics, gene regulation, and cancer biology.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ZMYND8 Polyclonal Antibody
SKU:
CAB21848
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RACK7, PRKCBP1, PRO2893, D8
Positive Sample:
HeLa, U-87 MG, A549, NIH/3T3
Cellular Localization:
Cytoplasm, Golgi Apparatus, Nucleoplasm, Nucleus.
Calculated MW:
132kDa
Observed MW:
165kDa
The protein encoded by this gene is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Finally, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
23613
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and ZMYND8 knockout (KO) HeLa cells, using [KO Validated] ZMYND8 Rabbit pAb (CAB21848) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of various lysates using [KO Validated] ZMYND8 Rabbit pAb (CAB21848) at 1:2000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
