Anti-ZMYND8 Antibody (CAB8737)

Antibody Name:	Anti-ZMYND8 Antibody [KO Validated]
Antibody SKU:	CAB8737
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 959-1188 of human ZMYND8 (NP_898868.1).

Application:	WB IHC
Recommended Dilution:	WB 1:1000 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87 MG, LO2, MCF-7, A375

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 959-1188 of human ZMYND8 (NP_898868.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LELT MAEM RQSL EQER DRLI AEVK KQLE LEKQ QAVD ETKK KQWC ANCK KEAI FYCC WNTS YCDY PCQQ AHWP EHMK SCTQ SATA PQQE ADAE VNTE TLNK SSQG SSSS TQSA PSET ASAS KEKE TSAE KSKE SGST LDLS GSRE TPSS ILLG SNQG SVSK RCDK QPAY APTT TDHQ PHPN YPAQ KYHS RSNK SSWS SSDE KRGS TRSD HNTS TSTK SLLP KESR LDTF WD
Gene ID:	23613
Uniprot:	Q9ULU4
Cellular Location:
Calculated MW:	66kDa/82kDa/109-138kDa
Observed MW:	165kDa

Synonyms:

ZMYND8, PRKCBP1, PRO2893, RACK7

Background:

The protein encoded by this gene is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Finally, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene.

UniProt Protein Function:	May act as a transcriptional corepressor for KDM5D. Required for KDM5D-mediated down-regulation of diverse metastasis-associated genes; the function seems to involve the recognition of the dual histone signature H3K4me1-H3K14ac. Suppresses prostate cancer cell invasion.
NCBI Summary:	The protein encoded by this gene is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Finally, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9ULU4
NCBI GenInfo Identifier:	528881103
NCBI Gene ID:	23613
NCBI Accession:	NP_001268700.1
UniProt Secondary Accession:	Q9ULU4,Q13517, Q2HXV1, B3KVL2, B7Z2A8, B7Z3E0, B7Z680 B7ZM62, E1P5U5, F5H0X3, H7C0U2, J3KPU3,
UniProt Related Accession:	Q9ULU4
Molecular Weight:	131 kDa
NCBI Full Name:	protein kinase C-binding protein 1 isoform e
NCBI Synonym Full Names:	zinc finger MYND-type containing 8
NCBI Official Symbol:	ZMYND8
NCBI Official Synonym Symbols:	RACK7; PRKCBP1; PRO2893
NCBI Protein Information:	protein kinase C-binding protein 1
UniProt Protein Name:	Protein kinase C-binding protein 1
UniProt Synonym Protein Names:	Cutaneous T-cell lymphoma-associated antigen se14-3; CTCL-associated antigen se14-3; Rack7; Zinc finger MYND domain-containing protein 8
UniProt Gene Name:	ZMYND8

	Western blot analysis of extracts of various cell lines, using ZMYND8 antibody (CAB8737) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Western blot analysis of extracts from normal (control) and ZMYND8 knockout (KO) HeLa cells, using ZMYND8 antibody (CAB8737) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Antibodies

ZMYND8 Antibody (PACO01392)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)