The [KO Validated] RAB27A Antibody (CAB1934) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reacts with samples from various species, including human, mouse, and rat. Validated for use in a range of experimental techniques, including Western blotting and immunofluorescence, this antibody enables precise detection and analysis of Rab27a protein levels in different cell types and tissues. Its reliability and specificity make it an essential tool for studies in cell biology, neurobiology, and cancer research.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
[KO Validated] RAB27A Antibody
SKU:
CAB1934
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GS2, RAM, RAB27, HsT18676, [KD Validated] RAB27A
Positive Sample:
U-87MG(KD)
Cellular Localization:
Late Endosome, Lipid-Anchor, Lysosome, Melanosome, Membrane.
Calculated MW:
25kDa
Observed MW:
27kDa
The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.
Purification Method
Affinity purification
Gene ID
5873
RRID
AB_2862644
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type(WT) and RAB27A knockdown (KD) U-87MG cells, using [KD Validated] RAB27A Rabbit pAb (CAB1934) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of HepG2 cells using [KD Validated] RAB27A Rabbit pAb (CAB1934) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
