Anti-ZNF346 Antibody (CAB13406)

Antibody Name:	Anti-ZNF346 Antibody
Antibody SKU:	CAB13406
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-294 of human ZNF346 (NP_036411.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	THP-1, HeLa, Mouse testis, Mouse brain, Rat lung, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-294 of human ZNF346 (NP_036411.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEYP APAT VQAA DGGA AGPY SSSE LLEG QEPD GVRF DRER ARRL WEAV SGAQ PVGR EEVE HMIQ KNQC LFTN TQCK VCCA LLIS ESQK LAHY QSKK HANK VKRY LAIH GMET LKGE TKKL DSDQ KSSR SKDK NQCC PICN MTFS SPVV AQSH YLGK THAK NLKL KQQS TKVE ALHQ NREM IDPD KFCS LCHA TFND PVMA QQHY VGKK HRKQ ETKL KLMA RYGR LADP AVTD FPAG KGYP CKTC KIVL NSIE QYQA HVSG FKHK NQSP KTVA SSLG QIPM QRQP IQKD STTL ED
Gene ID:	23567
Uniprot:	Q9UL40
Cellular Location:	Cytoplasm, Nucleus, nucleolus
Calculated MW:	29kDa/32kDa/35kDa
Observed MW:	38kDa

Synonyms:

ZNF346, JAZ, Zfp346

Background:

The protein encoded by this gene is a nucleolar, zinc finger protein that preferentially binds to double-stranded (ds) RNA or RNA/DNA hybrids, rather than DNA alone. Mutational studies indicate that the zinc finger domains are not only essential for dsRNA binding, but are also required for its nucleolar localization. The encoded protein may be involved in cell growth and survival. It plays a role in protecting neurons by inhibiting cell cycle re-entry via stimulation of p21 gene expression. Alternative splicing of this gene results in multiple transcript variants.

UniProt Protein Function:	ZNF346: Binds to dsRNA and DNA. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nucleolus Chromosomal Location of Human Ortholog: 5q35.2 Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus Molecular Function:protein binding; enzyme binding; zinc ion binding; double-stranded RNA binding Biological Process: positive regulation of apoptosis
NCBI Summary:	The protein encoded by this gene is a nucleolar, zinc finger protein that preferentially binds to double-stranded (ds) RNA or RNA/DNA hybrids, rather than DNA alone. Mutational studies indicate that the zinc finger domains are not only essential for dsRNA binding, but are also required for its nucleolar localization. The encoded protein may be involved in cell growth and survival. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UL40
NCBI GenInfo Identifier:	74735051
NCBI Gene ID:	23567
NCBI Accession:	Q9UL40.1
UniProt Secondary Accession:	Q9UL40,Q68CV9, Q6ZMW1, B7Z367,
UniProt Related Accession:	Q9UL40
Molecular Weight:	294
NCBI Full Name:	Zinc finger protein 346
NCBI Synonym Full Names:	zinc finger protein 346
NCBI Official Symbol:	ZNF346
NCBI Official Synonym Symbols:	JAZ; Zfp346
NCBI Protein Information:	zinc finger protein 346; just another zinc finger protein; double-stranded RNA-binding zinc finger protein JAZ
UniProt Protein Name:	Zinc finger protein 346
UniProt Synonym Protein Names:	Just another zinc finger protein
Protein Family:	Zinc finger protein
UniProt Gene Name:	ZNF346
UniProt Entry Name:	ZN346_HUMAN

	Western blot analysis of extracts of various cell lines, using ZNF346 antibody (CAB13406) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat brain using ZNF346 antibody (CAB13406) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human tonsil using ZNF346 antibody (CAB13406) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using ZNF346 antibody (CAB13406) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of NIH/3T3 cells using ZNF346 antibody (CAB13406) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-ZNF346 Antibody (CAB3397)

Anti-ZNF346 Antibody (CAB7507)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)