Western blot analysis of MCF7, using ZNF652 antibody (CAB22549) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The ZNF652 Polyclonal Antibody (CAB22549) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards ZNF652 in human samples, making it suitable for various applications such as Western blotting.ZNF652 is known for its involvement in regulating the expression of genes related to cell growth, differentiation, and development. By targeting ZNF652 with this antibody, researchers can gain insights into the molecular mechanisms underlying these processes, providing valuable information for studies in developmental biology, cancer research, and other fields.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ZNF652 Polyclonal Antibody
SKU:
CAB22549
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
MCF7
Cellular Localization:
Nucleus.
Calculated MW:
70kDa
Observed MW:
72kDa
Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be active in nucleus.
Purification Method
Affinity purification
Gene ID
22834
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from MCF7 cells, using ZNF652 Rabbit pAb (CAB22549) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
