The ZP3 Polyclonal Antibody (CAB8355) is a highly specific tool for researchers studying the zona pellucida glycoprotein 3 (ZP3) protein. This antibody is produced through immunization of rabbits and is optimized for use in various applications, including Western blotting and immunohistochemistry.ZP3 is an essential component of the zona pellucida, a glycoprotein matrix surrounding the mammalian oocyte. It plays a crucial role in sperm-egg interaction and fertilization. The ZP3 Polyclonal Antibody binds specifically to ZP3, allowing for detection and visualization of this protein in different cell types and tissues.
Research on ZP3 is particularly relevant in the field of reproductive biology and infertility studies. By understanding the function and regulation of ZP3, researchers can gain insights into mechanisms of fertilization and potential therapeutic targets for infertility treatments.Overall, the ZP3 Polyclonal Antibody is a valuable tool for investigating the role of ZP3 in reproductive processes and advancing our knowledge of fertility and assisted reproduction technologies.
Product Name:
ZP3 Rabbit Polyclonal Antibody
SKU:
CAB8355
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 170-390 of human ZP3 (NP_001103824.1).
Cell membrane, Secreted, Single-pass type I membrane protein, extracellular matrix, extracellular space.
Calculated MW:
47kDa
Observed MW:
47kDa
The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene.
Purification Method:
Affinity purification
Gene ID:
7784
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of SKOV3 cells, using ZP3 antibody (CAB8355) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
