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ZP3 Rabbit Polyclonal Antibody (CAB8355)

SKU:
CAB8355
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Developmental Biology
  • Developmental Biology Anti-ZP3 Antibody CAB8355
  • Developmental Biology Anti-ZP3 Antibody CAB8355
  • Developmental Biology Anti-ZP3 Antibody CAB8355
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:ZP3 Rabbit Polyclonal Antibody
SKU:CAB8355
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 170-390 of human ZP3 (NP_001103824.1).
Sequence:LTFS LRLM EENW NAEK RSPT FHLG DAAH LQAE IHTG SHVP LRLF VDHC VATP TPDQ NASP YHTI VDFH GCLV DGLT DASS AFKV PRPG PDTL QFTV DVFH FAND SRNM IYIT CHLK VTLA EQDP DELN KACS FSKP SNSW FPVE GSAD ICQC CNKG DCGT PSHS RRQP HVMS QWSR SASR NRRH VTEE ADVT VGPL IFLD RRGD HEVE QWAL PSDT SVVL L
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:ZPC; ZP3A; ZP3B; Zp-3; OOMD3; OZEMA3; ZP3
Positive Sample:SKOV3
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Secreted, Single-pass type I membrane protein, extracellular matrix, extracellular space.
Calculated MW:47kDa
Observed MW:47kDa

The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene.

Purification Method:Affinity purification
Gene ID:7784
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of SKOV3 cells, using ZP3 antibody (CAB8355) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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